Neumann, K: Plant Cell and Tissue Culture
(Sprache: Englisch)
With a detailed account of basic techniques and important applications alike, this book provides a general introduction as well as a selected survey of key advances in the fascinating field of plant cell and tissue culture as a tool in biotechnology.
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With a detailed account of basic techniques and important applications alike, this book provides a general introduction as well as a selected survey of key advances in the fascinating field of plant cell and tissue culture as a tool in biotechnology.
Klappentext zu „Neumann, K: Plant Cell and Tissue Culture “
From the contents: Historical Developments of Cell and Tissue Culture Techniques.- Callus Cultures.- Cell Suspension Cultures.- Protoplast Cultures.- Haploid Techniques.- Plant Propagation-Meristem Cultures,Somatic Embryogenesis.- Some Endogenous and Exogenous Factors in Cell Culture Systems.- Primary Metabolism.- Secondary Metabolism.- Phytohormones and Growth Regulators.- Cell Division, Cell Growth, Cell Differentiation.- Genetic Problems and Gene Technology.- Summary of Some Physiological Aspects in the Development of Plant Cell and Tissue Culture.- Summary: Applications of Plant Cell and Tissue Culture Systems.Inhaltsverzeichnis zu „Neumann, K: Plant Cell and Tissue Culture “
1 Introduction 2 Historical Developments of Cell and Tissue Culture Techniques
3 Callus Cultures
3.1 Establishment of a Primary Culture from Explants of the Secondary Phloem of the Carrot Root
3.2 Fermenter Cultures
3.3 Immobilized Cell Cultures
3.4 Nutrient Media
3.5 Evaluation of Experiments
3.6 Maintenance of Strains, Cryopreservation
3.7 Some Physiological, Biochemical, and Histological Aspects
4 Cell Suspension Cultures
4.1 Methods to Establish a Cell Suspension
4.2 Cell Population Dynamics
5 Protoplast Cultures
5.1 Production of Protoplasts
5.2 Protoplast Fusion
6 Haploid Techniques
6.1 Application Possibilities
6.2 Physiological and Histological Background
6.3 Methods for Practical Application
6.4 Haploid Plants
7 Plant Propagation-Meristem Cultures, Somatic Embryogenesis
7.1 General Remarks, and Meristem Cultures
7.2 Protocols of Some Propagation Systems
7.2.1 In Vitro Propagation of Cymbidium
7.2.2 Meristem Cultures of Raspberries
7.2.3 In Vitro Propagation of Anthurium
7.3 Somatic Embryogenesis
7.3.1 Basics of Somatic Embryogenesis
7.3.2 Ontogenesis of Competent Cells
7.3.3 Genetic Aspects-DNA Organization
7.3.4 The Phytohormone System
7.3.5 The Protein System
7.3.6 Cell Cycle Studies
7.4 Practical Application of Somatic Embryogenesis
7.5 Artificial Seeds
7.6 Embryo Rescue
8 Some Endogenous and Exogenous Factors in Cell Culture Systems
8.1 Endogenous Factors
8.1.1 Genetic Influences
8.1.2 Physiological Status of "Mother Tissue"
8.1.3 Growth Conditions of the "Mother Plant"
8.2 Exogenous Factors
8.2.1 Growth Regulators
8.2.2 Nutritional Factors
8.2.2.1 Improvement of Nutrient Uptake by Transgenic Carrot Cultures
8.3 Physical Factors
9 Primary Metabolism
9.1 Carbon Metabolism
9.2 Nitrogen Metabolism
10 Secondary Metabolism
10.1 Introduction
10.2 Mechanism of Production of Secondary Metabolites
10.3 Historical Background
10.4 Plant Cell Cultures and Pharmaceuticals, and Other
... mehr
Biologically Active Compounds
10.4.1 Antitumor Compounds
10.4.2 Anthocyanin Production
10.5 Strategies for Improvement of Metabolite Production
10.5.1 Addition of Precursors, and Biotransformations
10.5.2 Immobilization of Cells
10.5.3 Differentiation and Secondary Metabolite Production
10.5.4 Elicitation
10.5.4.1 Jasmonic Acid
10.5.4.2 Effect of UV on Production of Secondary Metabolites in Cultured Tissues
10.6 Organ Cultures
10.6.1 Shoot Cultures
10.6.2 Root Cultures
10.7 Genetic Engineering of Secondary Metabolites
10.8 Membrane Transport and Accumulation of Secondary Metabolites
10.9 Bioreactors
10.9.1 Technical Aspects of Bioreactor Systems
10.10 Prospects
11 Phytohormones and Growth Regulators
12 Cell Division, Cell Growth, Cell Differentiation
13 Genetic Problems and Gene Technology
13.1 Somaclonal Variations
13.1.1 Ploidy Stability
13.1.2 Some More Somaclonal Variations
13.2 Gene Technology
13.2.1 Transformation Techniques
13.2.1.1 Direct Gene Transfer
13.2.1.2 Agrobacterium-Mediated Gene Transformation
13.2.2 Selectable Marker Genes
13.2.2.1 Reporter Genes
13.2.2.2 Variants of GFP
13.2.3 ß-Glucuronidase (GUS)
13.2.3.1 Procedures for Assay of GUS Gene Expression
13.2.4 Antibiotics Resistance Genes
13.2.5 Elimination of Marker Genes
13.2.5.1 Cre-lox Recombination-Based Systems
13.2.5.2 Ac/Ds System
13.2.5.3 Double Cassette System
13.2.6 Agrobacterium-Mediated Transformation in Dicotyledonous Plants
13.2.6.1 Transgenic Carrot: Potential Source of Edible Vaccines
13.2.6.2 Cell Culture and Transformation Procedures
13.2.6.3 Uses of Transgenes to Increase Host Plant Resistance to Plant Pathogens
13.2.7 Agrobacterium-Mediated Transformation in Monocotyledonous Plants
13.2.7.1 Generation of Transgenic Barley Plants
14 Summary of Some Physiological Aspects in the Development of Plant Cell and Tissue Culture
15 Summary: Applications of Plant Cell and Tissue Culture Systems ReferencesMarker Genes
13.2.2.1 Reporter Genes
13.2.2.2 Variants of GFP
13.2.3 ß-Glucuronidase (GUS)
13.2.3.1 Procedures for Assay of GUS Gene Expression
13.2.4 Antibiotics Resistance Genes
13.2.5 Elimination of Marker Genes
13.2.5.1 Cre-lox Recombination-Based Systems
13.2.5.2 Ac/Ds System
13.2.5.3 Doubl
10.4.1 Antitumor Compounds
10.4.2 Anthocyanin Production
10.5 Strategies for Improvement of Metabolite Production
10.5.1 Addition of Precursors, and Biotransformations
10.5.2 Immobilization of Cells
10.5.3 Differentiation and Secondary Metabolite Production
10.5.4 Elicitation
10.5.4.1 Jasmonic Acid
10.5.4.2 Effect of UV on Production of Secondary Metabolites in Cultured Tissues
10.6 Organ Cultures
10.6.1 Shoot Cultures
10.6.2 Root Cultures
10.7 Genetic Engineering of Secondary Metabolites
10.8 Membrane Transport and Accumulation of Secondary Metabolites
10.9 Bioreactors
10.9.1 Technical Aspects of Bioreactor Systems
10.10 Prospects
11 Phytohormones and Growth Regulators
12 Cell Division, Cell Growth, Cell Differentiation
13 Genetic Problems and Gene Technology
13.1 Somaclonal Variations
13.1.1 Ploidy Stability
13.1.2 Some More Somaclonal Variations
13.2 Gene Technology
13.2.1 Transformation Techniques
13.2.1.1 Direct Gene Transfer
13.2.1.2 Agrobacterium-Mediated Gene Transformation
13.2.2 Selectable Marker Genes
13.2.2.1 Reporter Genes
13.2.2.2 Variants of GFP
13.2.3 ß-Glucuronidase (GUS)
13.2.3.1 Procedures for Assay of GUS Gene Expression
13.2.4 Antibiotics Resistance Genes
13.2.5 Elimination of Marker Genes
13.2.5.1 Cre-lox Recombination-Based Systems
13.2.5.2 Ac/Ds System
13.2.5.3 Double Cassette System
13.2.6 Agrobacterium-Mediated Transformation in Dicotyledonous Plants
13.2.6.1 Transgenic Carrot: Potential Source of Edible Vaccines
13.2.6.2 Cell Culture and Transformation Procedures
13.2.6.3 Uses of Transgenes to Increase Host Plant Resistance to Plant Pathogens
13.2.7 Agrobacterium-Mediated Transformation in Monocotyledonous Plants
13.2.7.1 Generation of Transgenic Barley Plants
14 Summary of Some Physiological Aspects in the Development of Plant Cell and Tissue Culture
15 Summary: Applications of Plant Cell and Tissue Culture Systems ReferencesMarker Genes
13.2.2.1 Reporter Genes
13.2.2.2 Variants of GFP
13.2.3 ß-Glucuronidase (GUS)
13.2.3.1 Procedures for Assay of GUS Gene Expression
13.2.4 Antibiotics Resistance Genes
13.2.5 Elimination of Marker Genes
13.2.5.1 Cre-lox Recombination-Based Systems
13.2.5.2 Ac/Ds System
13.2.5.3 Doubl
... weniger
Bibliographische Angaben
- Autoren: Karl-Hermann Neumann , Ashwani Kumar , Jafargholi Imani
- 2011, 333 Seiten, 154 Schwarz-Weiß-Abbildungen, Maße: 16 x 24,1 cm, Gebunden, Englisch
- Verlag: Springer
- ISBN-10: 3540938826
- ISBN-13: 9783540938828
Sprache:
Englisch
Rezension zu „Neumann, K: Plant Cell and Tissue Culture “
From the reviews:"The approach of including several examples and description of well-illustrated protocols from available primary literature and the author's own laboratory experiences and helpful guidelines make this volume a gem for conventional tissue culture practices. ... To the best of our experiences, this volume is suitable for both undergraduate and postgraduate level courses in life sciences, biotechnology, plant tissue culture, plant biotechnology ... or plant science courses. We also recommend this volume for M.Tech., M.Phil., and PhD level courses in the above-mentioned areas and related disciplines." (S. K. Basu and A. Goyal, Applied Biochemistry and Biotechnology, November, 2009)
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