Plant Cold Acclimation

Measuring Freezing Tolerance: Survival and Regrowth Assays.- Measuring Freezing Tolerance: Electrolyte Leakage and Chlorophyll Fluorescence Assays.- Conducting Field Trials for Freezing Tolerance Breeding in Cereals.- A Whole Plant Screening Test to Identify Genotypes with Superior Freezing Tolerance.- Mapping of Quantitative Trait Loci (QTL) Associated with Plant Freezing Tolerance and Cold Acclimation.- Common Garden Experiments to Characterize Cold Acclimation Responses in Plants from Different Climatic Regions.- Identification of Arabidopsis Mutants with Altered Freezing Tolerance.- Infrared Thermal Analysis of Plant Freezing Processes.- Cryo-Scanning Electron Microscopy to Study the Freezing Behavior of Plant Tissues.- Three-Dimensional Reconstruction of Frozen and Thawed Plant Tissues from Microscopic Images.- Proteomic Approaches to Identify Cold Regulated Soluble Proteins.- Proteomic Approaches to Identify Cold Regulated Plasma Membrane Proteins.- Profiling Methods to Identify Cold Regulated Primary Metabolites Using Gas Chromatography Coupled to Mass Spectrometry.- A Lipidomic Approach to Identify Cold-Induced Changes in Arabidopsis Membrane Lipid Composition.- Quantification of Superoxide and Hydrogen Peroxide in Leaves.- Estimating Ice Encasement Tolerance of Herbage Plants.- Characterization of Ice Binding Proteins from Sea Ice Algae.- Isolation and Characterization of Ice Binding Proteins from Higher Plants.