Plant Metabolic Flux Analysis

Application of Metabolic Flux Analysis to Plants.- Metabolic Network Reconstruction and Their Topological Analysis.- 14C pulse Labeling to Estimate External Fluxes and Turnovers in Primary Metabolism.- Optimization of Steady-state 13C-labeling Experiments for Metabolic Flux Analysis.- Quantification of 13C Enrichments and Isotopomer Abundances for Metabolic Flux analysis using 1D NMR Spectroscopy.- Analysis of Proteinogenic Amino Acid and Starch Labeling by 2D NMR.- Analysis of Kinetic Labeling of Amino Acids and Organic Acids by GC-MS.- Quantifying 13C-labeling in Free Sugars and Starch by GC-MS.- Liquid Chromatography Tandem Mass Spectrometry for Measuring 13C-labeling in Intermediates of the Glycolysis and Pentose-phosphate Pathway.- In Vivo NMR for 13C metabolic Flux Analysis.- Steady State and Instationary Modeling of Proteinogenic and Free Amino Acid Isotopomers for Flux Quantification.- Isotopically Nonstationary MFA (INST-MFA) of Autotrophic Metabolism.- Simulating Labeling to Estimate Kinetic Parameters for Flux Control Analysis.- High-throughput Data Pipelines for Metabolic Flux Analysis in Plants.- Analysis of Enzyme Activities.- Analytical Kinetic Modeling: A Practical Procedure.- Flux Balance Analysis as an Alternative Method to Estimate Fluxes Without Labeling.- Flux Variability Analysis: Application to Developing Oilseed Rape Embryos using Toolboxes for Constraint-Based Modeling.- Plant Genome-Scale Modeling and Implementation.- 34S and 15N Labelling to Model S and N Flux in Plants and Determine the Different Components of N and S use Efficiency.- Ecophysiological Process-based Model to Simulate Carbon Fluxes in Plants.