Pseudomonas Methods and Protocols
(Sprache: Englisch)
In Pseudomonas aeruginosa, expert researchers in the field detail many of the methods which are now commonly used to study this fascinating microorganism. Chapters include microbiological methods to high-throughput molecular techniques that have been...
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In Pseudomonas aeruginosa, expert researchers in the field detail many of the methods which are now commonly used to study this fascinating microorganism. Chapters include microbiological methods to high-throughput molecular techniques that have been developed over the last decade. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Pseudomonas aeruginosa aids in the continuing study of new and cutting edge findings.
Inhaltsverzeichnis zu „Pseudomonas Methods and Protocols “
Part I: BASIC PSEUDOMONAS FEATURES1. Gene Transfer: Transduction
Emanuela Frangipani
2. Gene Transfer: Transformation/Electroporation
Frédéric Cadoret, Chantal Soscia and Romé Voulhoux
3. Gene transfer: Conjugation
Thibault G. Sana, Aurélie Laubier and Sophie Bleves
4. Pseudomonas Bacteriophage Isolation and Production
Joana Azeredo, Sanna Sillankorva, and Diana Priscilla Pires
5. Genotyping Methods
Burkhard Tuemmler
6. Drug Susceptibility Testing by Dilution Methods
Katy Jeannot & Patrick Plesiat
7. Plate-based Assay for Swimming Motility in Pseudomonas aeruginosa
Dae-Gon Ha, Sherry L. Kuchma & George A. O'Toole
8. Plate-based Assay for Swarming Motility in Pseudomonas aeruginosa
Dae-Gon Ha, Sherry L. Kuchma and George A. O'Toole
9. Motility Assay: Twitching Motility
Lynne Turnbull and Cynthia B. Whitchurch
10. Qualitative and Quantitative Assays for Flagellum-mediated Chemotaxis
José-Antonio Reyes-Darias, Cristina García-Fontana, Andrés Corral Lugo, Miriam Rico-Jiménez & Tino Krell
11. Microscopic Analysis: Morphotypes and Cellular Appendages
Olga Zaborina, John Alverdy, Megha Shah, and Yimei Chen
Part II: PROTEINS AND PROTEIN TRANSPORT
12. Determination of Lipolytic Activities
Karl-Erich Jaeger and Filip Kovacic
13. Elastinolytic and Proteolytic Enzymes
Contributors: Efrat Kessler and Mary Safrin
14. In vitro Assays to Monitor the Activity of Pseudomonas aeruginosa Type III Secreted Proteins
Stéphanie L. Rolsma & Dara W. Frank
15. Cell Fractionation
Bérengère Ize, Véronique Viarr, and Romé Voulhoux
16. Characterization of Molecular Interactions using Isothermal Titration Calorimetry
Tino Krell, Jesús Lacal, Cristina Garcia-Fontana, Hortencia Silva-Jimenez, Miriam Rico-Jimenez, Andrés Corral Lugo, Jose A. Reyes Darias, and Juan Luis Ramos
17. Proteomic Analysis
Pascal Cosette and Thierry Jouenne
18. Membrane Proteomics of Pseudomonas
... mehr
aeruginosa
Zofia Magnowska, Isabel Hartmann, Lothar Jänsch and Dieter Jahn
19. Construction of Pseudomonas Two-hybrid Libraries for High-throughput Assays
Sophie de Bentzmann and Christophe Bordi
Part III: SMALL MOLECULES AND POLYSACCHARIDES
20. Biosensors for Qualitative and Semi-quantitative Analysis of Quorum Sensing Signal Molecules
Matthew Fletcher, Miguel Camara, David A. Barrett, and Paul Williams
21. LC-MS/MS Quantitative Analysis of Quorum Sensing Signal Molecules
Catherine A. Ortori, Nigel Halliday, Miguel Camara, Paul Williams, and David A. Barrett
22. LC/MS/MS-Based Quantitative Assay for the Secondary Messenger Molecule, c-di-GMP
Yasuhiko Irie and Matthew R. Parsek
23. Metabolic Footprinting: Extracellular Metabolomic Analysis
Volker Behrends, Huw D. Williams, and Jaco G. Bundy
24. Pyoverdine and Pyochelin Measurements
Françoise Hoegy, Gaetan L. A. Mislin & Isabelle J. Schalk
25. Measurement of Phenazines in Bacterial Cultures
Suzanne E. Kern, and Dianne K. Newman
26. Extraction and Measurement of NAD(P)+ and NAD(P)H
Suzanne E. Kern, Alexa Price-Whelan, and Dianne K. Newman
27. Cyanide Measurements in Bacterial Culture and Sputum
Chandrika Goh Nair, Ben Ryall, and Huw D. Williams
28. Monitoring Iron Uptake by Siderophores
Françoise Hoegy, and Isabelle J. Schalk
29. Exopolysaccharide Quantification
Irina Sadovskaya
30. Liquid Chromatography/Mass Spectrometry for the Identification and Quantification of Rhamnolipids
Ahmad Mohammad Abdel-Mawgoud, François Lépine & Eric Déziel
31. LPS quantitation Procedures
Joseph S. Lam, Erin M. Anderson, and Youai Hao
32. Monitoring Lectin Interaction with Carbohydrates
Sophie de Bentzmann, Annabelle Varrot, and Anne Imberty
Part IV: PSEUDOMONAS GENES and GENE EXPRESSION
33. Mining the Pseudomonas Genome
Geoffrey L. Winsor and Fiona S. L. Brinkman
34. Identification of Bacterial Small RNAs by RNA Sequencing
Maria Gomez-Lozano, Rasmus Lykke Marvig, Soeren Molin, and Katherine S. Long
35. Gene Amplification and qRT-PCR
Cerith Jones and Alain Filloux
36. The Standard European Vector architecture (SEVA) plasmid toolkit
Gonzalo Durante-Rodriguez, Victor de Lorenzo and Esteban Martinez-Garcia
37. Chromosomal integration of transcriptional fusions
Rafael Silva-Rocha and Victor de Lorenzo
38. A Method to Capture Large DNA Fragments from Genomic DNA
Geneviève Ball, Alain Filloux, and Romé Voulhoux
39. Transposon Mutagenesis
Hemantha D. Kulasekara and Bridget R. Kulasekara
40. Site-directed Mutagenesis and Gene Deletion using Reverse Genetics
Contributors: Daniela Muhl and Alain Filloux
41. Signature Tagged Mutagenesis
Irena-Kukavika-Ibrulj & Roger Levesque
42. Construction of a Pseudomonas aeruginosa Genomic Bank
Christophe Bordi
43. Strategy for Genome Sequencing Analysis and Assembly for Comparative Genomics of Pseudomonas Genomes
Julie Jeukens, Brian Boyle, Nicholas P. Tucker, and Roger Levesque
44. Promoter Fusions with Optical Outputs in Individual Cells and in Populations
Ilaria Benedetti and Victor de Lorenzo
45. Chromatin Immunoprecipitation for ChIP-chip and ChIP-Seq
Contributors: Sebastian Schulz and Susanne Haussler
46. Transcriptional Analysis of Pseudomonas aeruginosa Infected C.aenorhabditis elegans
Ashleigh MacKenzie, Lewis Stewart, Paul A. Hoskisson and Nicholas P. Tucker
Part V: LIFESTYLES AND INFECTION MODELS
47. Methods for Studying Biofilm Formation: Flow Cells and Confocal Laser Scanning Microscopy
Tim Tolker-Nielsen and Claus Sternberg
48. Biofilm Formation in the 96-well Microtiter Plate
Barbara M. Coffey and Gregory G. Anderson
49. Methods for Studying Biofilm Dispersal in Pseudomonas aeruginosa
Contributors: Nicolas Barraud, Joana A. Moscoso, Jean Marc Ghigo, and Alain Filloux
50. Pseudomonas aeruginosa PA14 Pathogenesis in Caenorhabditis Elegans
Natasha V. Kirienko, Brent O. Cezairliyan, Frederick M. Ausubel and Jennifer R. Powell
51. Assessing Pseudomonas Virulence using a Non-mammalian Host Model: Dictyostelium Discoideum
Geneviève Filion and Steve Charette
52. Assessing Pseudomonas virulence with non-mammalian host: Galleria mellonella
Gudrun Koch, Pol Nadal Jimenez, Robbert H. Cool &Wim J. Quax
53. Assessing Pseudomonas Virulence with the Non-mammalian Host: Arabidopsis Thaliana
Regina L. Baldini, Melissa Starkey, and Laurence G. Rahme
54. Assessing Pseudomonas aeruginosa Persister/Antibiotic Tolerant Cells
Ronen Hazan, Damien Maura, Yok-Ai Que & Laurence G. Rahme
55. Assessing Pseudomonas Virulence with Non-mammalian Host: Zebrafish
Maria A. Llamas and Astrid M. van der Sar
56. Assessing Pseudomonas Virulence with a Non-mammalian Host: Drosophila Melanogaster
Samantha Haller, Stefanie Limmer, and Dominique Ferrandon
57. Assessing Pseudomonas Virulence using Host Cells
Iwona Bucior, Cindy Tran, and Joanne Engel
58. Assessing Pseudomonas aeruginosa Virulence and the Host Response using Murine Models of Acute and Chronic Lung Infection
Irena Kukavica-Ibrulj, Marcella Facchini, Cristina Cigina, Roger Levesque & Alessandra Bragonzi
59. Assessing Pseudomonas Virulence using Mammalian Models: Acute Infection Model
Antje Munder and Burkhard Tuemmler
60. Burn Mouse Models
Henrik Calum, Niels Hoiby and Claus Moser
Zofia Magnowska, Isabel Hartmann, Lothar Jänsch and Dieter Jahn
19. Construction of Pseudomonas Two-hybrid Libraries for High-throughput Assays
Sophie de Bentzmann and Christophe Bordi
Part III: SMALL MOLECULES AND POLYSACCHARIDES
20. Biosensors for Qualitative and Semi-quantitative Analysis of Quorum Sensing Signal Molecules
Matthew Fletcher, Miguel Camara, David A. Barrett, and Paul Williams
21. LC-MS/MS Quantitative Analysis of Quorum Sensing Signal Molecules
Catherine A. Ortori, Nigel Halliday, Miguel Camara, Paul Williams, and David A. Barrett
22. LC/MS/MS-Based Quantitative Assay for the Secondary Messenger Molecule, c-di-GMP
Yasuhiko Irie and Matthew R. Parsek
23. Metabolic Footprinting: Extracellular Metabolomic Analysis
Volker Behrends, Huw D. Williams, and Jaco G. Bundy
24. Pyoverdine and Pyochelin Measurements
Françoise Hoegy, Gaetan L. A. Mislin & Isabelle J. Schalk
25. Measurement of Phenazines in Bacterial Cultures
Suzanne E. Kern, and Dianne K. Newman
26. Extraction and Measurement of NAD(P)+ and NAD(P)H
Suzanne E. Kern, Alexa Price-Whelan, and Dianne K. Newman
27. Cyanide Measurements in Bacterial Culture and Sputum
Chandrika Goh Nair, Ben Ryall, and Huw D. Williams
28. Monitoring Iron Uptake by Siderophores
Françoise Hoegy, and Isabelle J. Schalk
29. Exopolysaccharide Quantification
Irina Sadovskaya
30. Liquid Chromatography/Mass Spectrometry for the Identification and Quantification of Rhamnolipids
Ahmad Mohammad Abdel-Mawgoud, François Lépine & Eric Déziel
31. LPS quantitation Procedures
Joseph S. Lam, Erin M. Anderson, and Youai Hao
32. Monitoring Lectin Interaction with Carbohydrates
Sophie de Bentzmann, Annabelle Varrot, and Anne Imberty
Part IV: PSEUDOMONAS GENES and GENE EXPRESSION
33. Mining the Pseudomonas Genome
Geoffrey L. Winsor and Fiona S. L. Brinkman
34. Identification of Bacterial Small RNAs by RNA Sequencing
Maria Gomez-Lozano, Rasmus Lykke Marvig, Soeren Molin, and Katherine S. Long
35. Gene Amplification and qRT-PCR
Cerith Jones and Alain Filloux
36. The Standard European Vector architecture (SEVA) plasmid toolkit
Gonzalo Durante-Rodriguez, Victor de Lorenzo and Esteban Martinez-Garcia
37. Chromosomal integration of transcriptional fusions
Rafael Silva-Rocha and Victor de Lorenzo
38. A Method to Capture Large DNA Fragments from Genomic DNA
Geneviève Ball, Alain Filloux, and Romé Voulhoux
39. Transposon Mutagenesis
Hemantha D. Kulasekara and Bridget R. Kulasekara
40. Site-directed Mutagenesis and Gene Deletion using Reverse Genetics
Contributors: Daniela Muhl and Alain Filloux
41. Signature Tagged Mutagenesis
Irena-Kukavika-Ibrulj & Roger Levesque
42. Construction of a Pseudomonas aeruginosa Genomic Bank
Christophe Bordi
43. Strategy for Genome Sequencing Analysis and Assembly for Comparative Genomics of Pseudomonas Genomes
Julie Jeukens, Brian Boyle, Nicholas P. Tucker, and Roger Levesque
44. Promoter Fusions with Optical Outputs in Individual Cells and in Populations
Ilaria Benedetti and Victor de Lorenzo
45. Chromatin Immunoprecipitation for ChIP-chip and ChIP-Seq
Contributors: Sebastian Schulz and Susanne Haussler
46. Transcriptional Analysis of Pseudomonas aeruginosa Infected C.aenorhabditis elegans
Ashleigh MacKenzie, Lewis Stewart, Paul A. Hoskisson and Nicholas P. Tucker
Part V: LIFESTYLES AND INFECTION MODELS
47. Methods for Studying Biofilm Formation: Flow Cells and Confocal Laser Scanning Microscopy
Tim Tolker-Nielsen and Claus Sternberg
48. Biofilm Formation in the 96-well Microtiter Plate
Barbara M. Coffey and Gregory G. Anderson
49. Methods for Studying Biofilm Dispersal in Pseudomonas aeruginosa
Contributors: Nicolas Barraud, Joana A. Moscoso, Jean Marc Ghigo, and Alain Filloux
50. Pseudomonas aeruginosa PA14 Pathogenesis in Caenorhabditis Elegans
Natasha V. Kirienko, Brent O. Cezairliyan, Frederick M. Ausubel and Jennifer R. Powell
51. Assessing Pseudomonas Virulence using a Non-mammalian Host Model: Dictyostelium Discoideum
Geneviève Filion and Steve Charette
52. Assessing Pseudomonas virulence with non-mammalian host: Galleria mellonella
Gudrun Koch, Pol Nadal Jimenez, Robbert H. Cool &Wim J. Quax
53. Assessing Pseudomonas Virulence with the Non-mammalian Host: Arabidopsis Thaliana
Regina L. Baldini, Melissa Starkey, and Laurence G. Rahme
54. Assessing Pseudomonas aeruginosa Persister/Antibiotic Tolerant Cells
Ronen Hazan, Damien Maura, Yok-Ai Que & Laurence G. Rahme
55. Assessing Pseudomonas Virulence with Non-mammalian Host: Zebrafish
Maria A. Llamas and Astrid M. van der Sar
56. Assessing Pseudomonas Virulence with a Non-mammalian Host: Drosophila Melanogaster
Samantha Haller, Stefanie Limmer, and Dominique Ferrandon
57. Assessing Pseudomonas Virulence using Host Cells
Iwona Bucior, Cindy Tran, and Joanne Engel
58. Assessing Pseudomonas aeruginosa Virulence and the Host Response using Murine Models of Acute and Chronic Lung Infection
Irena Kukavica-Ibrulj, Marcella Facchini, Cristina Cigina, Roger Levesque & Alessandra Bragonzi
59. Assessing Pseudomonas Virulence using Mammalian Models: Acute Infection Model
Antje Munder and Burkhard Tuemmler
60. Burn Mouse Models
Henrik Calum, Niels Hoiby and Claus Moser
... weniger
Bibliographische Angaben
- 2014, 2014, XVIII, 816 Seiten, 89 farbige Abbildungen, Maße: 18,7 x 26 cm, Gebunden, Englisch
- Herausgegeben: Alain Filloux, Juan-Luis Ramos
- Verlag: Springer, Berlin
- ISBN-10: 1493904728
- ISBN-13: 9781493904723
Sprache:
Englisch
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