Regulatory Non-Coding RNAs

Diverse Functions and Mechanisms of Mammalian Long Non-Coding RNAs.- Sequence-Defined Oligoaminoamides for the Delivery of siRNAs.- A Biochemical Approach to Identify Direct MicroRNA Targets.- Native Gel Analysis for Mammalian MicroRNPs Assembled from Pre-MicroRNAs.- Identification and Validation of miRNA Target Sites within Non-Traditional miRNA Targets.- Fractionation of Non-Polyadenylated and Ribosomal-Free RNAs from Mammalian Cells.- Affinity-Purification of Long Noncoding RNA-Protein Complexes from Formaldehyde-Cross linked Mammalian Cells.- RNA Pull down Protocol for In Vitro Detection and Identification of RNA-Associated Proteins.- nRIP-seq: A Technique to Identify RNA Targets of an RNA Binding Protein on a Genome-Wide Scale.- Analysis of the Subcellular Distribution of RNA by Fluorescence In Situ Hybridization.- RNA Fluorescence In Situ Hybridization in Cultured Mammalian Cells.- Locating RNAs In Situ with FISH-STIC Probes.- Rapid and Efficient Elimination of Specific Nuclear Noncoding RNAs in Mammalian Cells with Antisense Oligonucleotides.- On Using Magnesium and Potassium Ions in RNA Experiments.- Studying the Affinity, Kinetic Stability and Specificity of RNA/Protein Interactions: SINE ncRNA/Pol II Complexes as a Model System.