RNA and DNA Editing

Three-Dimensional Reconstruction of Trypanosoma brucei Editosomes Using Single-Particle Electron Microscopy.- iCODA: RNAi-Based Inducible Knock-In System in Trypanosoma brucei.- Perturbing A-to-I RNA Editing Using Genetics and Homologous Recombination.- Laser Microdissection and Pressure Catapulting of Single Human Motor Neurons for RNA Editing Analysis.- Biochemical Identification of A-to-I RNA Editing sites by the Inosine Chemical Erasing (ICE) Method.- Identifying mRNA Editing Deaminase Targets by RNA-Seq.- Mouse and Other Rodent Models of C to U RNA Editing.- In vivo Analysis of RNA Editing in Plastids.- Identifying Specific Trans-Factors of RNA Editing in Plant Mitochondria by Multiplex Single Base Extension Typing.- Complementation of Mutants in Plant Mitochondrial RNA Editing by Protoplast Transfection.- A High Throughput Assay for DNA Deaminases.- Biochemical Fractionation and Purification of High-Molecular-Mass APOBEC3G Complexes.- Analysis of tRNA Editing in Native and Synthetic Substrates.- Post-Transcriptional Modification of RNAs by Artificial Box H/ACA and Box C/D RNPs.- Functional Analysis of Non-Coding RNAs in Trypanosomes: RNA Walk, A Novel Approach to Study RNA-RNA Interactions Between Small RNA and Its Target.- A Post-Labeling Approach for the Characterization and Quantification of RNA Modifications Based on Site-Directed Cleavage by DNAzymes.

From the reviews:

"This book on RNA and DNA Editing, Methods and Protocols provides methods to study editing substrates, mechanisms of specificity and functions of RNA and DNA editing enzymes and complexes ... . The apparent audience is molecular biologists interested in RNA and DNA editing." (Omer Iqbal, Doody's Book Reviews, March, 2012)"It is quite timely that a Methods book dedicated at experimental approaches to investigate RNA editing mechanisms should find its rightful place in the series. To achieve this aim, the editor ... has successfully united many leading experts in this field to write several Chapters dedicated at explaining state-of-the-art techniques ... . this is an invaluable store of information that can potentially save weeks or months spent simply in learning what-to-do and what-not-to-do when setting up a particular procedure in their own lab." (Emanuele Buratti, Molecular Biotechnology, September, 2011)

From the reviews:

"This book on RNA and DNA Editing, Methods and Protocols provides methods to study editing substrates, mechanisms of specificity and functions of RNA and DNA editing enzymes and complexes ... . The apparent audience is molecular biologists interested in RNA and DNA editing." (Omer Iqbal, Doody's Book Reviews, March, 2012)"It is quite timely that a Methods book dedicated at experimental approaches to investigate RNA editing mechanisms should find its rightful place in the series. To achieve this aim, the editor ... has successfully united many leading experts in this field to write several Chapters dedicated at explaining state-of-the-art techniques ... . this is an invaluable store of information that can potentially save weeks or months spent simply in learning what-to-do and what-not-to-do when setting up a particular procedure in their own lab." (Emanuele Buratti, Molecular Biotechnology, September, 2011)