Single-Domain Antibodies

 Nanobodies: From Serendipitous Discovery of Heavy Chain-Only Antibodies in Camelids to a Wide Range of Useful Applications.- Overview, Generation, and Significance of Variable New Antigen Receptors (VNARs) as a Platform for Drug and Diagnostic Development.- Llama DNA Immunization and Isolation of Functional Single-Domain Antibody Binders.- Preparation of Immune and Synthetic VNAR Libraries as Sources of High-Affinity Binders.-  Isolation of Single-Domain Antibodies to Transmembrane Proteins Using Magnetized Yeast Cell Targets.- A Transgenic Heavy Chain IgG Mouse Platform as a Source of High Affinity Fully Human Single-Domain Antibodies for Therapeutic Applications.- Cytoplasmic Production of Nanobodies and Nanobody-Based Reagents by Co-Expression of Sulfhydryl Oxidase and DsbC Isomerase.- Small Scale Secretory VHH Expression in Saccharomyces cerevisiae.- Production of Single-Domain Antibodies in Pichia pastoris.- Production of Designer VHH-Based Antibodies in Plant.- Assessing the Aggregation Propensity of Single-Domain Antibodies Upon Heat-Denaturation Employing the DeltaTm Shift.- Facile Affinity Maturation of Single-Domain Antibodies Using Next-Generation DNA Sequencing.- Engineering pH-Sensitive Single-Domain Antibodies.- Humanization of Camelid Single-Domain Antibodies.- Creation of Multimeric Single-Domain Antibodies Using Bacterial Superglues.- Introducing Cysteines into Nanobodies for Site-Specific Labelling.- Affinity-Guided Site Selective Labelling of Nanobodies With Aldehyde Handles.- Cytoplasmic Expression of Nanobodies with Formylglycine Generating Enzyme Tag and Conversion to a Bio-Orthogonal Aldehyde Group.- Site-Specific Fluorescent Labelling, Single-Step Immunocytochemistry, and Delivery of Nanobodies into Living Cells.- Design and Validation of Site-Specifically Labelled Single-Domain Antibody-Based Tracers forIn Vivo Fluorescence Imaging and