Stem Cell Mobilization
Methods and Protocols
(Sprache: Englisch)
This book provides detailed state-of-the-art protocols for analyzing individual aspects of stem cell mobilization at the molecular and cellular level with contributions encompassing the most recent technological developments. Specifically, the volume covers...
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Klappentext zu „Stem Cell Mobilization “
This book provides detailed state-of-the-art protocols for analyzing individual aspects of stem cell mobilization at the molecular and cellular level with contributions encompassing the most recent technological developments. Specifically, the volume covers cell adhesion and migration assays in three-dimensional models, imaging techniques for the determination of blood flow or hypoxic status, innovative biomechanical studies of stem cells, and assays to determine the involvement of proteases or complement factors, as well as other key topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stem Cell Mobilization: Methods and Protocols is an ideal guide for all laboratories in their endeavors to decipher the mechanisms of stem cell mobilization, ultimately helping stem cell donors and patients around the world.
Inhaltsverzeichnis zu „Stem Cell Mobilization “
1. Evolution of Peripheral Blood Stem Cell TransplantationAnthony D. Ho
2. In Vitro Dynamic Phenotyping for Testing Novel Mobilizing Agents
Motomu Tanaka 3. Colony Formation: An Assay of Hematopoietic Progenitor Cells
Romy Kronstein-Wiedemann and Torsten Tonn
4. Mobilization and Collection of Peripheral Blood Stem Cells in Adults: Focus on Timing and Benchmarking
Katharina Kriegsmann and Patrick Wuchter
5. Transmigration Assays for the Determination of Molecular Interactions between Hematopoietic Stem Cells and Niche Cells
Reinhard Henschler and Rudolf Richter
6. Microfluidic Shear Force Assay to Determine Cell Adhesion Forces
Julia Hümmer, Julian Koc, Axel Rosenhahn, and Cornelia Lee-Thedieck
7. A Microcavity Array-Based 3D-Model System of the Hematopoietic Stem Cell Niche
Eric Gottwald, Cordula Nies, Patrick Wuchter, Rainer Saffrich, Roman Truckenmüller, and Stefan Giselbrecht
8. Migration Assay for Leukemic Cells in a 3D Matrix toward a Chemoattractant
Sabrina Zippel, Annamarija Raic, and Cornelia Lee-Thedieck
9. Intravital Imaging of Blood Flow and HSPC Homing in Bone Marrow Microvessels
Jonas Stewen and Maria Gabriele Bixel
10. Assessing Cellular Hypoxic Status In Situ within the Bone Marrow Microenvironment
Ute Suessbier and César Nombela-Arrieta
11. Analysis of Biomechanical Properties of Hematopoietic Stem and Progenitor Cells Using Real-Time Fluorescence and Deformability Cytometry
Angela Jacobi, Philipp Rosendahl, Martin Kräter, Marta Urbanska, Maik Herbig, and Jochen Guck
12. Assessment of Proteolytic Activities in the Bone Marrow Microenvironment
Andreas Maurer, Gerd Klein, and Nicole D. Staudt
13. Analysis of the Complement Cascade Activation during Mobilization of Hematopoietic Stem/Progenitor Cells
Anna I. Grabowska and Jakub
... mehr
M. Hawryluk
14. A Freezing Protocol for Hematopoietic Stem Cells
Petra Pavel and Sascha Laier
15. Assessment of Young and Aged Hematopoietic Stem Cell Activity by Competitive Serial Transplantation Assays
Yu Wei Zhang and Nina Cabezas Wallscheid
16. Zebrafish Xenografts for the In Vivo Analysis of Healthy and Malignant Human Hematopoietic Cells
Martina Konantz, Joëlle S. Müller, and Claudia Lengerke
17. Statistical and Mathematical Modeling of Spatiotemporal Dynamics of Stem Cells
Walter de Back, Thomas Zerjatke, and Ingo Roeder
14. A Freezing Protocol for Hematopoietic Stem Cells
Petra Pavel and Sascha Laier
15. Assessment of Young and Aged Hematopoietic Stem Cell Activity by Competitive Serial Transplantation Assays
Yu Wei Zhang and Nina Cabezas Wallscheid
16. Zebrafish Xenografts for the In Vivo Analysis of Healthy and Malignant Human Hematopoietic Cells
Martina Konantz, Joëlle S. Müller, and Claudia Lengerke
17. Statistical and Mathematical Modeling of Spatiotemporal Dynamics of Stem Cells
Walter de Back, Thomas Zerjatke, and Ingo Roeder
... weniger
Bibliographische Angaben
- 2019, 1st ed. 2019, XI, 248 Seiten, 54 farbige Abbildungen, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Gerd Klein, Patrick Wuchter
- Verlag: Springer, Berlin
- ISBN-10: 1493995731
- ISBN-13: 9781493995738
- Erscheinungsdatum: 14.06.2019
Sprache:
Englisch
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