Stem Cells

Preface to the Professor xvii
 
Preface to the Student xxi
 
Acknowledgments xxiii
 
List of Figures xxv
 
List of Tables xxxvii
 
List of Case Studies xxxix
 
List of Focus Boxes xli
 
1 A HISTORY OF STEM CELL RESEARCH 1
 
Early Studies 1
 
Hematopoietic Stem Cell Discovery 4
 
Mouse Embryonic Stem Cell Discovery 6
 
Successful Neural Stem Cell Culture 7
 
The Discovery of Cancer Stem Cells 8
 
Human Embryonic Stem Cell Discovery 9
 
Stem Cells And Cloning 11
 
Cord Blood Embryonic?]Like Stem Cells--An Alternative to Es and Adult Stem Cells 14
 
Breakthrough In Spinal Cord Injury Repair 15
 
The Generation of ips Cells 16
 
iPS Cells Derived from Keratinocytes 20
 
iPS Induction Without the Use of Viruses 20
 
Transposon?]Mediated iPS 21
 
Protein?]Based iPS 22
 
The Discovery of Human Amniotic Stem Cells 24
 
Human Embryonic Stem Cells Generated Without Embryo Destruction 25
 
Human Cloning 25
 
Mesenchymal Stem Cell?]Derived Human Knee Cartilage 27
 
The First Clinical Trial Using Human Embryonic Stem Cells 28
 
Mitochondrial DNA: A Barrier To Autologous Cell Therapeutics 29
 
Induced Pluripotency And The Potential To Save Endangered Species 30
 
Chapter Summary 33
 
Key Terms 37
 
Review Questions 39
 
Thought Question 40
 
Suggested Readings 40
 
2 FUNDAMENTALS OF STEM CELLS 43
 
Basic in Vitro Cell Culture--A Historical Perspective 43
 
Stem Cell Culture--Optimal Conditions and Techniques 48
 
Embryonic Stem Cell Culture 49
 
Hematopoietic Stem Cell Culture 52
 
Notch Regulation of HSC Proliferation 52
 
Other Drivers of HSC Proliferation 53
 
Adipose?]Derived Stem Cell Culture 54
 
The Study of Embryonic Development 56
 
Embryonic Development and the Origin of