Synthetic Biology

What is Synthetic Biology?.- Gene Synthesis Method Based on Overlap Extension PCR and DNAWorks Program.- BioBrick(TM) Assembly Using the In-Fusion PCR Cloning Kit.- Overlap Extension PCR Cloning.- One-Step Isothermal Assembly of DNA Fragments.- Creation and Characterization of Component Libraries for Synthetic Biology.- In vivo and in vitro Characterisation of s70 Constitutive Promoter BioParts by Real-time PCR and Fluorescent Measurements.- In Vivo Screening of Artificial Small RNAs for Silencing Endogenous Genes in Escherichia coli.- Construction and Engineering of Large Biochemical Pathways via DNA Assembler.- Assembly of Multi-gene Pathways and Combinatorial Pathway Libraries through ePathBrick Vectors.- Tandem Recombineering by SLIC Cloning and Cre-LoxP Fusion to Generate Multigene Expression Constructs for Protein Complex Research.- Combinatorial DNA Assembly using Golden Gate Cloning.- Construction of Synthetic Gene Circuits in the Escherichia coli Genome.- Shuffling of DNA Cassettes in a Synthetic Integrin.- Systematic Methodology for the Development of Mathematical Models for Biological Processes.-SOBOLHDMR: A General Purpose Modelling Software.