The Mitotic Spindle

Using FluorescenceMicroscopy to Study Mitosis.- Using PhotoactivatableGFP to Study Microtubule Dynamics and Chromosome Segregation.- Purification and Fluorescent Labelingof Tubulin from Xenopus Laevis Egg Extracts.- Measuringthe Effects of Microtubule-Associated Proteins on Microtubule Dynamics In Vitro.- Imagingand Quantifying the Dynamics of Gamma-Tubulin at Microtubule Minus Ends in Mitotic Spindles.- Visualizing and Analyzing Branching Microtubule Nucleation Using Meiotic Xenopus Egg Extracts and TIRF Microscopy.- Encapsulationof Xenopus Egg and Embryo Extract SpindleAssembly Reactions in Synthetic Cell-Like Compartmentswith Tunable Size.- In Vitro Kinetochore Assembly.- Biochemicaland Structural Analysis of Kinetochore Histone-Fold Complexes.- Measuring Kinetochore-Microtubule Attachment Stability in Cultured Cells.- StudyingKinetochores In Vivo Using FLIM-FRET.- Purification of Fluorescently LabeledSaccharomyces Cerevisiae Spindle PoleBodies.- A Cell-FreeSystem for Real-Time Analyses of Centriole Disengagement and Centriole to CentrosomeConversion.- Assaysto Study Mitotic Centrosome and Spindle Pole Assembly and Regulation.- AnalyzingSpindle Positioning Dynamics in Cultured Cells.- Quantification of Mitotic Chromosome Alignment.- Imaging Mitosisin the Moss Physcomitrella Patens.- Small MoleculeApproach to Study the Function of Mitotic Kinesins.- Identificationand Characterization of Mitotic Spindle-Localized Transcripts.- Probing Mitosisby Manipulating the Interactions of Mitotic Regulator Proteins Using Rapamycin-InducibleDimerization.- StudyingKinetochore Kinases.- Engineeringand Functional Analysis of Mitotic Kinases through Chemical Genetics.- Using CellCulture Models of Centrosome Amplification to Study Centrosome Clustering in Cancer.- Generationand Purification of Tetraploid Cells.- Anti-MicrotubuleDrugs.