Thermogenic Fat

Isolation, Primary Culture, and Differentiation of Preadipocytes from Mouse Brown Adipose Tissue.- Isolation of Mouse Stromal Vascular Cells for Monolayer Culture.- Flow Cytometry Assisted Isolation of Adipose Tissue Derived Stem Cells.- Flow Cytometric Isolation and Differentiation of Adipogenic Progenitor Cells into Brown and Brite/Beige Adipocytes.- Immuno-Magnetic Isolation and Thermogenic Differentiation of White Adipose Tissue Progenitor Cells.- Isolation of Immune Cells from Adipose Tissue.- Differentiation and Metabolic Interrogation of Human Adipocytes.- Protocols for Generation of Immortalized Human Brown and White Preadipocyte Cell Lines.- Gene Expression and Histological Analysis of Activated Brown Adipocytes in Adipose Tissue.- Genetic Mouse Models: The Powerful Tools to Study Fat Tissues.- Genetic Manipulation with Viral Vectors to Assess Metabolism and Adipose Tissue Function.- Bioenergetic Analyses in Adipose Tissue.- Oxygen Consumption Rate and Energy Expenditure in Mice: Indirect Calorimetry.- Isolation and Patch-Clamp of Primary Adipocytes.- In Vitro Approaches to Model and Study Communications Between Adipose Tissue and the Liver.- Identification and Quantification of Human Brown Adipose Tissue.- Designing 3D Adipospheres for Quantitative Metabolic Study.- Culture and Sampling of Primary Adipose Tissue in Practical Microfluidic Systems.- Using Thermogenic Beige Cells to Identify Biologically Active Small Molecules and Peptides.