Transgenic Mouse Methods and Protocols

Table of Contents Preface Contributors 1. Introduction: Strategies for developing genetically modified mice Marten H. Hofker 2. Genetic Modification of the Mouse; General Technology: Pronuclear and Blastocyst Injection Jan Willem Vonken 3. Generation of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos Jérôme Artus and Anna-Katerina Hadjantonakis 4. Cryopreservation of mouse spermatozoa and In vitro fertilization Naomi Nakagata 5. Autopsy and histological analysis of the transgenic mouse Marion J.J. Gijbels and Menno P.J. de Winther 6. Transgene design Bart van de Sluis and Jan Willem Vonken 7. Inducible Transgenic Mouse Models Thomas L. Saunders 8. Lentiviral transgenesis Terunaga Nakagawa and Casper C. Hoogenraad 9. Transgenesis in Mouse Embryonic Stem Cells Janine van Ree, Wei Zhou, Ming Li and Jan van Deursen 10. Engineering BAC Reporter Gene Constructs for Mouse Transgenesis Yu Fu and Peter Maye 11. Targeting Vector Construction Through Recombineering Liviu A. Malureanu 12. Generating Conditional Knockout Mice Roland H. Friedel, Wolfgang Wurst, Benedikt Wefers and Ralf Kühn 13. Hypomorphic mice Daren J. Baker 14. MICER Targeting Vectors for Manipulating the Mouse Genome Chunhong Liu, Paul F. Szurek and Y. Eugene Yu 15. Knockin approaches Anton J.M. Roebroek, Philip L.S.M. Gordts and Sara Reekmans 16. Generation of a series of knockin alleles using RMCE in ES cells Anton J.M. Roebroek, Philip L.S.M. Gordts and Sara Reekmans 17. Selection of targeted mutants from a library of randomly mutagenized ES cells Kyoji Horie, George Gaitanaris, and Alexander Gragerov 18. Generation of genetically modified rodents using random ENU mutagenesis Ruben van Boxtel and Edwin Cuppen 19. Bone marrow transplantations to study gene function in hematopoietic cells Menno P.J. de Winther and Peter Heeringa 20. Adenovirus Mediated Gene Transfer Ko Willems van Dijk, Kyriakos E. Kypreos, Frits J. Fallaux and Jurre Hageman

From the reviews of the second edition: "Provided us with the valuable second edition of Transgenic mouse ... . A great merit of this book is then the fact that the two Editors decided to devote some chapters to this basic topic! ... I strongly suggest to have a copy of this book to those would like to enter the fascinating field of transgenesis; those already active in the field will anyhow surely find interesting aspects to deal with by comparing the techniques they are using with the suggested novelties here presented." (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 55, 2011)

From the reviews of the second edition:"Provided us with the valuable second edition of Transgenic mouse ... . A great merit of this book is then the fact that the two Editors decided to devote some chapters to this basic topic! ... I strongly suggest to have a copy of this book to those would like to enter the fascinating field of transgenesis; those already active in the field will anyhow surely find interesting aspects to deal with by comparing the techniques they are using with the suggested novelties here presented." (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 55, 2011)