Ubiquitin Family Modifiers and the Proteasome

Three Decades of Studies to Understand the Functions of the Ubiquitin Family.- Structure and Evolution of Ubiquitin and Ubiquitin-Related Domains.- Deciphering Tissue-Specific Ubiquitylation by Mass Spectrometry.- Analysis of Cellular SUMO and SUMO-Ubiquitin Hybrid Conjugates.- Recombinant Reconstitution of Sumoylation Reactions In vitro.- Reconstitution of SUMO-Dependent Ubiquitylation In vitro.- Detection and Analysis of FAT10 Modification.- Isolation of NEDDylated Proteins in Human Cells.- The ISG15 Conjugation System.- Pupylation: Proteasomal Targeting by a Protein Modifier in Bacteria.- Role of UbF Family Modifiers and their Binding Proteins in Cell Signaling.- Isolation of Ubiquitylated Proteins using Tandem Ubiquitin-Binding Entities (TUBEs).- Using Linkage-Specific Monoclonal Antibodies to Analyze Cellular Ubiquitination.- Measuring Rates of Ubiquitin Chain Formation as a Functional Readout of Ligase Activity.- Synthesis and Analysis of K11-Linked Ubiquitin Chains.- Synthesis and Analysis of Linear Ubiquitin Chains.- Detection and Quantitation of SUMO Chains by Mass Spectrometry.- Bioinformatical Detection of Recognition Factors for Ubiquitin and SUMO.- Surface Plasmon Resonance to Measure Interactions of UbFs with Their Binding Partners.- Identifying and Studying Ubiquitin Receptors by NMR.- Exploring the Role of p97 and its UBX-Domain Cofactors Through Identification of Their Interacting Proteins.- Assembly and Function of the Proteasome.- Using Native Gel Electrophoresis and Phosphofluoroimaging to Analyse GFP-tagged Proteasomes.- Disulfide Engineering to Map Subunit Interactions in the Proteasome and Other Macromolecular Complexes.- Using DNA Damage Sensitivity Phenotypes to Characterize Mutations Affecting Proteasome Function.- Analysing Properties of Proteasome Inhibitors using Kkinetic and X-Ray Crystallographic Studies.- Immunoproteasome-Specific Inhibitors and Their Application.- Binding of Ubiquitin Conjugates to Proteasomes as Visualized with