Vibrio Cholerae

Laboratory Culturing Techniques and Maintenance of Vibrio cholera.- Genotypic and Phenotypic Assays to Distinguish Vibrio cholera Biotype.- Preparation of Vibrio cholera Samples for RNA-seq Analysis.- Random Transposon Mutagenesis of Vibrio cholera.- Metabolomics of Vibrio cholera.- Natural Cotransformation and Multiplxed Genome Editing by Natural Transformation (MuGENT) of Vibrio cholera.- Vibrio cholera Chromatin Immunoprecipitation.- Fly Models of Vibrio cholera Infection and Colonization.- Danio rerio as a Native Host Model for Understanding Pathophysiology of Vibrio cholera.- Transposon Sequencing of Vibrio cholera in the Infant Rabbit Model of Cholera.- Isolation of Outer Membrane Vesicles Including their Quantitative and Qualitative Analyses.- Utilization of Vibrio cholera as a Model Organism to Screen natural Product Libraries for Identification of New Antibodies.- Infant Mouse Model of Vibrio cholera Infection and Colonization.- Methods for Assessments of Collagenolytic Activity of the Vibrio cholera Extracellular Proteases, Purification of Secreted Collagenase VchC, and Extraction of Type I Collagen from Fish Skin.- Proteomics of Vibrio cholerarea.