Whole Genome Amplification
Methods and Protocols
(Sprache: Englisch)
This volume mirrors the holistic feature of whole genome amplification (WGA) technology by combining reviews, detailed basic methods and advanced sample workflows. The first part of the book covers an overview of the development of WGA techniques throughout...
Jetzt vorbestellen
versandkostenfrei
Buch (Gebunden)
109.99 €
- Lastschrift, Kreditkarte, Paypal, Rechnung
- Kostenlose Rücksendung
- Ratenzahlung möglich
Produktdetails
Produktinformationen zu „Whole Genome Amplification “
Klappentext zu „Whole Genome Amplification “
This volume mirrors the holistic feature of whole genome amplification (WGA) technology by combining reviews, detailed basic methods and advanced sample workflows. The first part of the book covers an overview of the development of WGA techniques throughout recent years including general considerations on bias in WGA, possible sample pre-enrichment strategies and how to run a single-cell lab. The second part focuses on major WGA methods and protocols that allow the assessment of WGA product quality. The final chapters contain advanced protocols and address issues such as sample preparation using laser-micro dissection; WGA from partially degraded DNA (formalin-fixed paraffin embedded samples); circulating tumor cells; and ancient samples. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Authoritative and thorough, Whole Genome Amplification: Methods and Protocols will serve as a rich source of detailed information and inspiration and will help researchers, both new and experienced, yield successful results.
Inhaltsverzeichnis zu „Whole Genome Amplification “
1. Principles of Whole Genome AmplificationZbigniew Tadeusz Czyz, Stefab Kirsch, and Bernhard Polzer
2. Bias in Whole Genome Amplification: Causes and Considerations
Jeffrey X. Sabina and John H. Leamon
3. The Single-Cell Lab or How to Perform Single-Cell Molecular Analysis
Roland Kirchner and Marianna Alunni-Fabbroni
4. Sample Preparation Methods Following CellSearch Approach Compatible of Single-Cell Whole Genome Amplification: An Overview
Joost F. Swennenhuis and Leon W.M.M. Terstappen
5. Deterministic Whole Genome Amplification of Single Cells
Zbigniew T. Czyz and Christoph A. Klein
6. Construction of a DNA Library on Microbeads Using Whole Genome Amplification
Kojima T., Zhu B., and Nakano H.
7. Heat-Induced Fragmentation and Adapter-Assisted Whole Genome Amplification Using GenomePlex® Single Cell Whole Genome Amplification Kit (WGA4)
Amin El-Heliebi, Shukun Chen, and Thomas Kroneis
8. Whole Genome Amplification by Isothermal Multiple Strand Displacement Using Phi29 DNA Polymerase
Thomas Kroneis and Amin El-Heliebi
9. Using Multiplex-PCR for Assessing the Quality of Whole Genome Amplified DNA
Amin El-Heliebi, Shukun Chen, and Thomas Kroneis
10. Quality Control of Isothermal Amplified DNA Based on Short Tandem Repeat Analysis
Thomas Kroneis and Amin El-Heliebi
11. Laser-Microdissection of FFPE Tissue Areas and Subsequent Whole Genome Amplification by Ampli1TM
Zbigniew T. Czyz, Nikolas H. Stoecklein, and Bernhard Polzer
12. Whole Genome Amplification from Blood Spot Samples
Karina Meden Sørensen
13. Analysis of Whole Mitogenomes from Ancient Samples
Gloria G. Fortes and Johanna L.A. Paijmans
14. Copy Number Variation Analysis by Array Analysis of Single Cells Following Whole Genome Amplification
Eftychia Dimitriadou, Masoud Zamani Esteki, and Joris Robert Vermeesch
15. Whole Genome Amplification in Genomic Analysis of Single Circulating Tumor Cells
Christin Gasch, Klaus Pantel, and Sabine
... mehr
Riethdorf
16. Whole Genome Amplification of Labeled Viable Single Cells Suited for Array Comparative Genomic Hybridization
Thomas Kroneis and Amin El-Heliebi
17. Low-Volume On-Chip Single-Cell Whole Genome Amplification for Multiple Subsequent Analyses
Thomas Kroneis, Shukun Chen, and Amin El-Heliebi
18. Detection and Characterization of Circulating Tumor Cells by the CellSearch Approach
Frank Coumans and Leon W.M.M. Terstappen
16. Whole Genome Amplification of Labeled Viable Single Cells Suited for Array Comparative Genomic Hybridization
Thomas Kroneis and Amin El-Heliebi
17. Low-Volume On-Chip Single-Cell Whole Genome Amplification for Multiple Subsequent Analyses
Thomas Kroneis, Shukun Chen, and Amin El-Heliebi
18. Detection and Characterization of Circulating Tumor Cells by the CellSearch Approach
Frank Coumans and Leon W.M.M. Terstappen
... weniger
Autoren-Porträt
DI Dr. Thomas Kroneis Medical University Graz Institute of Cell Biology, Histology & Embryology Head of Research Unit for Single Cell Analysis +43(0)316/380-4236 mailto: thomas.kroneis@medunigraz.at
Bibliographische Angaben
- 2015, 1st ed. 2015, X, 284 Seiten, 13 farbige Abbildungen, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Thomas Kroneis
- Verlag: Springer, Berlin
- ISBN-10: 1493929895
- ISBN-13: 9781493929894
- Erscheinungsdatum: 16.09.2015
Sprache:
Englisch
Kommentar zu "Whole Genome Amplification"
Schreiben Sie einen Kommentar zu "Whole Genome Amplification".
Kommentar verfassen