Mass Spectrometry in Biophysics / Wiley-Interscience Series on Mass Spectrometry (PDF)
Conformation and Dynamics of Biomolecules
(Sprache: Englisch)
The first systematic summary of biophysical mass spectrometry
techniques
Recent advances in mass spectrometry (MS) have pushed the frontiers
of analytical chemistry into the biophysical laboratory. As a
result, the biophysical community's acceptance...
techniques
Recent advances in mass spectrometry (MS) have pushed the frontiers
of analytical chemistry into the biophysical laboratory. As a
result, the biophysical community's acceptance...
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The first systematic summary of biophysical mass spectrometry
techniques
Recent advances in mass spectrometry (MS) have pushed the frontiers
of analytical chemistry into the biophysical laboratory. As a
result, the biophysical community's acceptance of MS-based methods,
used to study protein higher-order structure and dynamics, has
accelerated the expansion of biophysical MS.
Despite this growing trend, until now no single text has presented
the full array of MS-based experimental techniques and strategies
for biophysics. Mass Spectrometry in Biophysics expertly closes
this gap in the literature.
Covering the theoretical background and technical aspects of each
method, this much-needed reference offers an unparalleled overview
of the current state of biophysical MS. Mass Spectrometry in
Biophysics begins with a helpful discussion of general biophysical
concepts and MS-related techniques. Subsequent chapters
address:
* Modern spectrometric hardware
* High-order structure and dynamics as probed by various MS-based
methods
* Techniques used to study structure and behavior of non-native
protein states that become populated under denaturing
conditions
* Kinetic aspects of protein folding and enzyme catalysis
* MS-based methods used to extract quantitative information on
protein-ligand interactions
* Relation of MS-based techniques to other experimental tools
* Biomolecular properties in the gas phase
Fully referenced and containing a helpful appendix on the physics
of electrospray mass spectrometry, Mass Spectrometry in Biophysics
also offers a compelling look at the current challenges facing
biomolecular MS and the potential applications that will likely
shape its future.
techniques
Recent advances in mass spectrometry (MS) have pushed the frontiers
of analytical chemistry into the biophysical laboratory. As a
result, the biophysical community's acceptance of MS-based methods,
used to study protein higher-order structure and dynamics, has
accelerated the expansion of biophysical MS.
Despite this growing trend, until now no single text has presented
the full array of MS-based experimental techniques and strategies
for biophysics. Mass Spectrometry in Biophysics expertly closes
this gap in the literature.
Covering the theoretical background and technical aspects of each
method, this much-needed reference offers an unparalleled overview
of the current state of biophysical MS. Mass Spectrometry in
Biophysics begins with a helpful discussion of general biophysical
concepts and MS-related techniques. Subsequent chapters
address:
* Modern spectrometric hardware
* High-order structure and dynamics as probed by various MS-based
methods
* Techniques used to study structure and behavior of non-native
protein states that become populated under denaturing
conditions
* Kinetic aspects of protein folding and enzyme catalysis
* MS-based methods used to extract quantitative information on
protein-ligand interactions
* Relation of MS-based techniques to other experimental tools
* Biomolecular properties in the gas phase
Fully referenced and containing a helpful appendix on the physics
of electrospray mass spectrometry, Mass Spectrometry in Biophysics
also offers a compelling look at the current challenges facing
biomolecular MS and the potential applications that will likely
shape its future.
Autoren-Porträt von Igor A. Kaltashov, Stephen J. Eyles
IGOR A. KALTASHOV, PhD, is currently an Assistant Professor in theDepartment of Chemistry at the University of Massachusetts,
Amherst.
STEPHEN J. EYLES, PhD, is a Lecturer in the Department of
Polymer Science and Engineering and the Director of Mass
Spectrometry and Molecular Weight Characterization Facility at the
University of Massachusetts, Amherst.
Bibliographische Angaben
- Autoren: Igor A. Kaltashov , Stephen J. Eyles
- 2005, 1. Auflage, 320 Seiten, Englisch
- Verlag: John Wiley & Sons
- ISBN-10: 0471705160
- ISBN-13: 9780471705161
- Erscheinungsdatum: 21.04.2005
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