Quantitation of mRNA by Polymerase Chain Reaction / Springer Lab Manuals (PDF)
Nonradioactive PCR Methods
(Sprache: Englisch)
In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques for quantifying nucleic acids in biological and clincial samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based...
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In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques for quantifying nucleic acids in biological and clincial samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quanatitative PCR protocols into their own clinical or biomedical research.
Bibliographische Angaben
- Autoren: Thomas Köhler , Dirk Laßner , Anne-Katrin Rost , Barbara Thamm , Barbara Pustowoit , Harald Remke
- 2012, 1995, 166 Seiten, Englisch
- Verlag: Springer Berlin Heidelberg
- ISBN-10: 3642797121
- ISBN-13: 9783642797125
- Erscheinungsdatum: 06.12.2012
Abhängig von Bildschirmgröße und eingestellter Schriftgröße kann die Seitenzahl auf Ihrem Lesegerät variieren.
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- Dateiformat: PDF
- Größe: 14 MB
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Englisch
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