Reduction of Bioburden by Optimizing Gamma Dose for Enhancing Export Value of Pakistani Peaches (PDF)

Master's Thesis from the year 2014 in the subject Chemistry - Bio-chemistry, grade: A, , course: Biotechnology, language: English, abstract: Peach is the most commonly eaten stone fruit which grows in the temperate regions of
the world. These are also among the most exported fruits in term of volume and value.
Peaches, being perishable and susceptible to microbial spoilage, have a short shelflife.

The goal of this study was to determine the effect of gamma irradiation on the
microbiological and organoleptic properties of peaches and secondly, to determine the
effects of irradiation on shelf-life of the fruit. The fruit (local variety, No.4), at proper
maturity stage was collected and irradiated with 0.25, 0.5, and 0.75kGy dose, stored
under refrigerated (4
o
C) conditions for a period of three weeks. Total viable count,
Gram negative Enterobacteriaceae count, Salmonella-Shigella count, yeast and mold
count, loss in weight and decay percentage was evaluated after 7, 14 and 21 days of
storage (4
o
C). Data obtained from microbiological and sensory evaluations of
irradiated peaches was compared with the observations obtained from unirradiated
(control) samples. Microbial evaluation of the fruits revealed the presence of
Salmonella sonnie on SS agar and non fermentor spp. on macConkey agar. Ten types
of yeasts were isolated on potato dextrose agar and analyzed microscopically. Studies
showed total bacterial, yeast and molds significantly decreased with increasing dose
level. The sensory evaluation of the fruits revealed that irradiation preserves the
texture and appearance of fruit. The gamma-irradiation dose of 0.75kGy proved to be
effective in reducing weight loss and significantly (p=0.05) delaying the decaying of
the fruit by 7 days under refrigerated (4
o
C) conditions. Statistical studies were carried
out using Duncan's multiple range test (DMRT) and means were separated by LSD at
5 %. It is hoped that this particular type of research will help in improving export
quality of peaches.