Single Particle Tracking and Single Molecule Energy Transfer (PDF)

Preface PART I: Single-Particle Imaging and Tracking THREE DIMENSIONAL PARTICLE TRACKING IN A LASER SCANNING FLUORESCENCE MICROSCOPE Introduction Image-Based Single-Particle Tracking Methods Advanced Fluorescence Microscopy Techniques for Single-Particle Tracking Two-Photon Excitation Microscopy 3-D Tracking in Image-Based SPT Approaches 3-D Tracking in Laser Scanning Microscopes Instrumentation Background Noise Simultaneous Two-Particle Tracking Application: Chromatin Dynamics in Interphase Cells Conclusions THE TRACKING OF INDIVIDUAL MOLECULES IN CELLS AND TISSUES Introduction Single-Molecule and Single-Particle Localization Positional Accuracy Tracking Trajectory Analysis Applications Conclusions MESSENGER RNA TRAFFICKING IN LIVING CELLS Intranuclear Structure and Dynamics FCS and FRAP Studies of Nuclear mRNP Mobility Single-Particle Tracking of mRNA Molecules Single-Particle Tracking of Specific, Native mRNPs In Vivo Labeling of Native BR2 mRNPs Outlook: Light Sheet-Based Single-Molecule Microscopy QUANTUM DOTS: INORGANIC FLUORESCENT PROBES FOR SINGLE-MOLECULE TRACKING EXPERIMENTS IN LIVE CELLS Introduction Fluorescent Labels for Single-Molecule Tracking in Cells Optical Properties of Colloidal Quantum Dots Synthesis of Colloidal Fluorescent Quantum Dots Surface Chemistry for the Water-Solubilization of Quantum Dots Interfacing Quantum Dots with Biology Single Quantum Dot Tracking Experiments in Live Cells Conclusions and Perspectives PART II: Energy Transfer on the Nanoscale SINGLE-PAIR FRET: AN OVERVIEW WITH RECENT APPLICATIONS AND FUTURE PERSPECTIVES Introduction Principles of FRET spFRET in Solution spFRET on Immobilized Molecules Future Prospects ALTERNATING-LASER EXCITATION AND PULSED-INTERLEAVED EXCITATION OF SINGLE MOLECULES Introduction ALEX: The Principles of Operation MyALEX Nanosecond-ALEX/Pulsed Interleaved Excitation (PIE) msALEX Three-Color ALEX Conclusions and Outlook UNRAVELING THE DYNAMICS BRDIGING PROTEIN STRUCTURE AND FUNCTION ONE MOLECULE AT

Christoph Bräuchle is Professor at the Ludwig-Maximilian-University (LMU) Munich. After his PhD, he spent one year as a postdoc at IBM in San José, California, USA. His research focuses on imaging, spectroscopy and manipulation of single molecules in bio- and nano-sciences. Besides numerous publications in international journals, Prof. Bräuchle has won several honors, including the prestigious Philip Morris Research award and the Karl Heinz Beckurts Prize 2002. He also is a member of the Academia Europaea.

Don C. Lamb is Professor for Biophysical Chemistry at the LMU Munich.
He received his PhD from the University of Illinois at Urbana-Champaign and was a research fellow at the Harvard Medical School, an Alexander von Humboldt Research Fellow at the TU Munich, a member of the Laboratory for Fluorescence Dynamics at the University of Illinois at Urbana-Champaign and a visiting scientist at the University of Ulm. His research centers around ultra-sensitive fluorescence methods, single molecule studies, protein function and dynamics, fluorescence fluctuation spectroscopies, live-cell imaging, single particle tracking and single virus tracing.

Jens Michaelis is an Assistant Professor at the LMU Munich. After receiving his PhD in physics in 2000, he spent several years as a postdoc at the University of California, Berkley, focusing on single-molecule studies of molecular motors. His research interests are the molecular mechanisms underlying the biological activity of proteins and mechanical properties of polymer molecules. In 2007 he was awarded the Römer Prize from the LMU for his habilitation work.

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