Advanced Technologies for Protein Complex Production and Characterization

Protein complex production from the drug discovery standpoint.- Choose a suitable expression host: a survey of available protein production platforms.- ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts.- Complex reconstitution and characterization by combining co-expression techniques in Escherichia coli with high-throughput.- Membrane protein production in E. coli for applications in drug discovery.- Cell-free Synthesis of Macromolecular Complexes.- A Bacillus megaterium system for the production of recombinant proteins and protein complexes.- Protein complex production in alternative prokaryotic hosts.- Production of Protein Complexes in Non-methylotrophic and Methylotrophic Yeasts.- Leishmania tarentolae for the Production of Multi-Subunit Complexes.- Alternative Eukaryotic Expression Systems for the Production of Proteins and Protein Complexes.- Fundamentals of baculovirus expression and applications.- The MultiBac Baculovirus / Insect Cell Expression Vector System for Producing Complex Protein Biologics.- Fundamentals of expression in mammalian cells.- Assembling multi-subunit complexes using mammalian expression.- Microalgae as solar-powered protein factories.- Strategies and methodologies for the co-expression of multiple proteins in plants.- Transient expression systems in plants-potentialities and constraints.- Complex reconstitution from individual protein modules.- Structural reconstruction of protein-protein complexes involved in intracellular signaling.- The use of small-angle scattering for the characterization of multi subunit complexes.- Application of nuclear magnetic resonance and hybrid methods to structure determination of complex systems.   

Dr. M. Cristina Vega is a group leader at the Chemical and Physical Biology Department of the Center for Biological Research (CIB-CSIC) in Madrid, Spain. With substantial experience in macromolecular X-ray crystallography of protein complexes with biomedical relevance, she designs eukaryotic, yeast-based expression platforms tailored to protein complexes with a focus on cost-efficiency and improved performance.