Allergy

Understanding of the Molecular Mechanisms of Allergy.- T-Cell: Primary Culture from Peripheral Blood.- Measurement of Allergen-Specific Inhibitory Antibody Activity.- Immunoelectrophoresis for the Characterization of Allergen Extracts.- Standardization of Allergen Extracts.- Quantifying Human Innate Cytokine and Chemokine Responses Ex Vivo via Pattern Recognition Receptor Stimulation.- Improved Methods for Quantifying Human Chemokine and Cytokine Biomarker Responses: Ultrasensitive ELISA and Meso Scale Electrochemiluminescence Assays.- Development of Mould Antigen-Specific Enzyme-Linked Immunosorbent Assays (ELISA) to Quantify Airborne Antigen Exposure.- Monocyte-Derived Dendritic Cells as Antigen Presenting Cells in T-Cell Proliferation and Cytokine Production.- The Use of Dual Cell Tracker Dye Staining for the Identification and Characterization of Peanut Specific T-Cell Subsets.- The Use of pMHCII Tetramer Technology for the Identification and Characterization of CD4+ T Cells.- Isolation and Characterization of Lymphocytes from Human Mucosal Biopsies.- Development of a Dipstick Assay for Soy Allergens: Inexpensive Detection to Control Allergen Exposure.- Novel Method for the Purification of House Dust Mite Allergen Der p 1 and Its Use in Structure-Based Chemical Design of Novel Inhibitors.- Measurement of Specific IgG Anti-Fel d 1 Antibodies.- Measurement of Specific IgG4 Anti-Mouse Urine Antibodies.- Preparation of Samples for a Mass Spectrometry Based Method to Identify Allergenic Proteins.

"This well-edited book with contributions by recognized experts is an important resource for bench researchers in this field. ... The expertly written, detailed laboratory protocols with illustrations and specific notes should serve as a ready reference enabling investigators to achieve successful completion of the various determinations." (Donald F. German, Doody's Book Reviews, November 08, 2019)