Analysis of Nucleic Acids by Capillary Electrophoresis
(Sprache: Englisch)
Die Kapillarelektrophorese (CE) ist heute in biochemischen Laboratorien noch nicht als Routinemethode zur DNA-Trennung etabliert, obwohl sie diverse Vorzüge gegenüber den derzeitigen Methoden besitzt.
In diesem Band stellen verschiedene Wissenschaftler...
In diesem Band stellen verschiedene Wissenschaftler...
Voraussichtlich lieferbar in 3 Tag(en)
versandkostenfrei
Buch (Kartoniert)
109.99 €
- Lastschrift, Kreditkarte, Paypal, Rechnung
- Kostenlose Rücksendung
- Ratenzahlung möglich
Produktdetails
Produktinformationen zu „Analysis of Nucleic Acids by Capillary Electrophoresis “
Die Kapillarelektrophorese (CE) ist heute in biochemischen Laboratorien noch nicht als Routinemethode zur DNA-Trennung etabliert, obwohl sie diverse Vorzüge gegenüber den derzeitigen Methoden besitzt.
In diesem Band stellen verschiedene Wissenschaftler die theoretischen Aspekte der Trennung von Oligonucleinsäuren mit der CE, die instrumentellen Grundlagen und deren Grenzen und schließlich die praktische Anwendung in der Biochemie und Molekularbiologie vor.
This book on capillary electrophoresis is unique in its focus on the separation of nucleic acids. The importance of electrophoretic separation in every molecular biology laboratory justifies this specialization, which is also reflected in the development of instrumentation.
This book is aimed to help to implement this rather new and promising technology in the biological laboratories and to help to overcome typical problems that can occur when starting with a new technique. It should also trigger further development in the field. It covers the theoretical background as well as practical examples of usual applications.
The authors are all experts in their field, having many years of experience with capillary electrophoresis and their application to nucleic acids.
In diesem Band stellen verschiedene Wissenschaftler die theoretischen Aspekte der Trennung von Oligonucleinsäuren mit der CE, die instrumentellen Grundlagen und deren Grenzen und schließlich die praktische Anwendung in der Biochemie und Molekularbiologie vor.
This book on capillary electrophoresis is unique in its focus on the separation of nucleic acids. The importance of electrophoretic separation in every molecular biology laboratory justifies this specialization, which is also reflected in the development of instrumentation.
This book is aimed to help to implement this rather new and promising technology in the biological laboratories and to help to overcome typical problems that can occur when starting with a new technique. It should also trigger further development in the field. It covers the theoretical background as well as practical examples of usual applications.
The authors are all experts in their field, having many years of experience with capillary electrophoresis and their application to nucleic acids.
Klappentext zu „Analysis of Nucleic Acids by Capillary Electrophoresis “
During the last decade, capillary electrophoresis has been developed into a very powerful analytical technique, which has many advantages over conventional slab gel techniques. The improvement is comparable to the one occuring earlier in the field of chromatography, with the introduction of the high performance technology. How important this technique has be come, is reflected by the shear volume of papers published each month; as well as a dozen of books already published on this subject. One of the most important conferences in the field, the "International Symposium on High Performance Capillary Electrophoresis" attracts now nearly thousand people every year. As capillary electrophoresis can be applied to many different analytical problems, a spe cialization is unavoidable. This evolution is also reflected in the development of instrumen tation: whereas the first devices were designed for all possible applications, new instruments are now built, that are specialized for one particular task, e.g. DNA analysis. I very much welcome the decision of the series editor and the publisher, to edit a series ofspecialized books, covering all aspects of capillary electrophoresis. Having worked on the electrophoretic separation of DNA for many years, I am convinced that there are so many different aspects on this issue that they deserve a whole book on their own. Therefore, I was happy to agree when being asked to edit this book.
Inhaltsverzeichnis zu „Analysis of Nucleic Acids by Capillary Electrophoresis “
I Basic Concepts.- 1 The Separation Matrix.- 2 Electrophoresis Theories.- 3 Microscopic Studies on the Migration Mechanism.- II Factors Affecting the Separation.- 4 Influence of Polymer Concentration and Electric Field.- 5 Effects of Sample Matrix and Injection.- 6 Developments in Capillary Coating and DNA Separation Matrices.- III Applications.- 7 Analysis of Oligonucleotides and Antisense DNA Analogs.- 8 Separation of DNA Restriction Fragments and PCR Products.- 9 Pulsed-Field Capillary Electrophoresis of Large DNA.- 10 Capillary Electrophoresis for DNA Sequencing.- 11 Mutational Analysis with Capillary Electrophoresis.- 12 Analysis of Transfer RNA and 5S Ribosomal RNA.- IV Recent Developments.- 13 Update on Improvements in DNA Separation.
Autoren-Porträt
Dr. Christoph Heller ist Wissenschaftler am Max-Planck-Institut für molekulare Genetik in Berlin.The editor, Dr. Christoph Heller, is currently working at the Max-Planck-Institute for Molecular Genetics in Berlin-Dahlem, Germany. Since 1985 he is working in the field of DNA separation, both in conventional gels and - since five years - also in capillaries.
Bibliographische Angaben
- 2013, Softcover reprint of the original 1st ed. 1997, X, 314 Seiten, Maße: 14,8 x 21 cm, Kartoniert (TB), Englisch
- Herausgegeben: Christoph Heller, Kevin D. Altria
- Verlag: Vieweg+Teubner
- ISBN-10: 3322910172
- ISBN-13: 9783322910172
Sprache:
Englisch
Kommentar zu "Analysis of Nucleic Acids by Capillary Electrophoresis"
Schreiben Sie einen Kommentar zu "Analysis of Nucleic Acids by Capillary Electrophoresis".
Kommentar verfassen