Barley

An Introduction to Barley: The Crop and the Model.- Mutation Breeding in Barley:  Historical Overview.- A Practical Guide to Barley Crossing.- Barley Anther Culture.- Isolated Microspore Culture in Barley.- TILLING in Barley.- Virus-Induced Gene Silencing (VIGS) for Functional Characterization of Disease Resistance Genes in Barley Seedlings.- Agrobacterium-Mediated Transformation of Barley Immature Embryos.- Methods for the Simple and Reliable Assessment of Barley Sensitivity to Abiotic Stresses during Early Development.- Preparation of Barley Roots for Histological, Structural, and Immunolocalization Studies Using Light and Electron Microscopy.- Preparation of Barley Pollen Mother Cells for Confocal and Super Resolution Microscopy.- Microarrays for High-Throughput Gene Expression Analysis of Barley.- Genome Engineering Using TALENs.- Creating Targeted Gene Knock-Outs in Barley Using CRISPR/Cas9.- Genotyping-by-Sequencing on the Ion Torrent Platform in Barley.- DNA Methylation Analysis in Barley and Other Species with Large Genomes.- High Resolution RT-PCR Analysis of Alternative Barley Transcripts.- Exome Capture for Variant Discovery and Analysis in Barley.