BCL-2 Family Proteins

Overview of BCL-2 Family Proteins and Therapeutic Potentials.- Database and Bioinformatic Analysis of BCL-2 Family Proteins and BH3-Only Proteins.- Application of Mito-Priming to Generate BCL-2 Addicted Cells.- BH3 Profiling: A Functional Assay to Measure Apoptotic Priming and Dependencies.- Flow Cytometry-Based Detection and Analysis of BCL-2 Family Proteins and Mitochondrial Outer Membrane Permeabilization (MOMP).- Investigating BCL-2 Family Protein Interactions in Yeast.- Liposomal Permeabilization Assay to Study the Functional Interaction of the BCL-2 Family.- Mitochondrial Isolation, Gel Filtration, and Real-Time Monitoring of MOMP.- Photocrosslinking Approach to Investigate Protein Interactions in the BCL-2 Family.- Assessment of Dynamic BCL-2 Protein Shuttling between Outer Mitochondrial Membrane and Cytosol.- Quantification of BCL-2 Family Members by Flow Cytometry.- Methods to Probe Calcium Regulation by BCL-2 FamilyMembers.- Methods to Probe Conformational Activation and Mitochondrial Activity of Proapoptotic BAK.- Probing BAK and BAX Activation and Pore Assembly with Cytochrome c Release, Limited  Proteolysis and Oxidant-Induced Linkage.- BCL-2 Protein Family Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy.- Reconstitution and Characterization of BCL-2 Family Proteins in Lipid Bilayer Nanodiscs.- Cryo-Electron Microscopy to Study Bax Pores and MOMP.- CW EPR and DEER Methods to Determine BCL-2 Family Protein Structure and Interactions: Application of Site-Directed Spin Labeling to BAK Apoptotic Pores.- Rapid Imaging of BCL-2 Family Interactions in Live Cells using FLIM-FRET.- Quantification of the Interactions between BCL-2 Proteins by Fluorescence Correlation Spectroscopy.- Isolation of Synthetic Antibodies against BCL-2-Associated X Protein (BAX).