Beta-Arrestins

Preface... Table of Contents... Contributing Authors... Part I Introduction 1. A Brief History of the b-Arrestins Marc G. Caron and Lawrence S. Barak 2. b-Arrestins: Multitask Scaffolds Orchestrating the Where and When In Cell Signaling Stéphane A. Laporte and Mark G.H. Scott Part II Methods to Study Roles of b-Arrestins in GPCR Regulation 3. Methods to Monitor the Trafficking of ß-Arrestin/G Protein-Coupled Receptor Complexes using Enhanced Bystander BRET Yubo Cao, Yoon Namkung, and Stéphane A. Laporte 4. Methods to Investigate b-Arrestin-Mediated Regulation of GPCR Function in Human Airway Smooth Muscle Tonio Pera and Raymond B. Penn 5. Measuring Recruitment of b-Arrestin to G Protein-Coupled Heterodimers using Bioluminescence Resonance Energy Transfer Dany Fillion, Dominic Devost, and Terence E. Hébert 6. Detection of ß-Arrestin-Mediated G Protein-Coupled Receptor Ubiquitination using BRET Karim Nagi and Sudha K. Shenoy Part III Methods to Study Scaffolding Functions of b-Arrestins 7. Using In Vitro Pull-Down and In Cell Overexpression Assays to Study Protein Interactions with Arrestin Nicole A. Perry, Xuanzhi Zhan, Eugenia V. Gurevich, T. M. Iverson, and Vsevolod V. Gurevich 8. Methods to Investigate Arrestins in Complex with Phosphodiesterases Amy Tibbo, Tara Busiau, and George S. Baillie 9. Methods to Characterize Protein Interactions with ß-arrestin in cellulo Revu Ann Alexander, Isaure Lot, and Hervé Enslen 10. Methods to Investigate the ß-Arrestin-Mediated control of ARF6 Activation to Regulate Trafficking and Actin Cytoskeleton Remodeling Ricardo Charles, Mohamed Bourmoum, Shirley Campbell, and Audrey Claing 11. Methods to Investigate the Roles of b-Arrestin-Dependent RalGDS Activation in GPCR-Stimulated Membrane Blebbing Stephen S. G. Ferguson 12. Methods to Determine Interaction Interfaces between b-Arrestins and Their Protein Partners Thomas Bourquard, Astrid Musnier, Aurélie Tréfier, Flavie Landomiel, Thomas Boulo, Eric Reiter, Pascale Crépieux, and