Bone Research Protocols
Methods and Protocols
(Sprache: Englisch)
The third edition of this book provides a large number of laboratory protocols that are intended for basic and clinical bone researchers. Chapters are organized into five parts covering methods for isolation, ex vivo models of bone organ and cell co-culture...
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The third edition of this book provides a large number of laboratory protocols that are intended for basic and clinical bone researchers. Chapters are organized into five parts covering methods for isolation, ex vivo models of bone organ and cell co-culture system, biochemical procedures, animal models of bone remodeling, and rodent models on Rheumatoid arthritis and osteoarthritis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge Bone Research Protocols: Methods and Protocols, Third Edition aims to extensive list of protocols for anyone interested in bone research.
Inhaltsverzeichnis zu „Bone Research Protocols “
Part I: In Vitro Culture of Bone Cells1. Isolation and Generation of Osteoclasts
Nadia Rucci, Alberta Zallone, and Anna Teti
2. Isolation and Generation of Osteoblasts Inês P Perpétuo, Lucie E Bourne, and Isabel R Orriss 3. Isolation, Purification, Generation, and Culture of Osteocytes
Jonathan H Gooi, Ling Yeong Chia, Christina Vrahnas, and Natalie A Sims
4. Isolation, Differentiation, and Characterisation of Human Bone Marrow Stem Cells In Vitro and In Vivo
Janos Kanczler, Rahul S. Tare, Peter D. Mitchell, Patrick Stumpf, Timothy J. Noble, Cameron Black, and Richard O.C. Oreffo
5. 2d and 3d In Vitro Co-Culture For Cancer and Bone Cell Interaction Studies
Silvia Marino, Ryan T. Bishop, Daniëlle De Ridder, Jesus Delgado-Calle, and Michaela R Reagan
6. Using Cell and Organ Culture Models to Analyse Responses of Bone Cells to Mechanical Stimulation
Soraia P Caetano-Silva, Astrid Novicky, Behzad Javaheri, Simon Cf Rawlinson, and Andrew A Pitsillides
Part II: Ex Vivo Culture of Bone Cells
7. The ex vivo Organ Culture of Bone
Katherine A. Staines, Genevieve Brown, and Colin Farquharson
8. Ex-Vivo Model Systems of Cancer-bone Cell Interactions
Erdjan Salih 9. Mechanical Properties of Bone ex vivo
Simon R. Goodyear and Richard M. Aspden
Part III: Ex Vivo Culture of Bone Cells 10. Analysis of Signalling Pathways by Western Blotting and Immunoprecipitation
Silvia Marino and Aymen I. Idris 11. Analysis of Transcriptional Regulation in Bone Cells
Huilin Jin, Antonia Sophocleous, Asim Azfer, and Stuart H Ralston
12. Analysis of mRNA, miRNA, and DNA in bone cells by RT-qPCR and In situ Hybridization
Brice Moukengue, Jérôme Amiaud, Camille Jacques, Céline Charrier, Benjamin Ory, and Francois Lamoureux
Part IV: In Vivo Models of Bone 13. Ovariectomy/Orchiectomy in
... mehr
Rodents Antonia Sophocleous and Aymen I. Idris
14. In Vivo Models of Rheumatoid Arthritis Bettina Grötsch, Aline Bozec and Georg Schett 15. Osteoarthritis Mouse Model of Destabilisation of the Medial Meniscus
Antonia Sophocleous and Carmen Huesa 16. Models of Prostate Cancer Bone Metastasis
Sun Hee Park, Matthew Robert Eber, andYusuke Shiozawa
17. Animal Models of Breast Cancer Bone Metastasis
Claudia Tulotta, Arwin Groenewoud, B. Ewa Snaar-Jagalska, and Penelope Ottewell
18. Murine Models of Bone Sarcomas
Camille Jacques, Nathalie Renema, Benjamin Ory,Carl R. Walkley, Agi E. Grigoriadis, and Dominique Heymann
19. Mouse Models of Melanoma Bone Metastasis
Özge Uluçkan
20. Animal Models of Multiple Myeloma
Sam W.Z. Olechnowicz and Claire M. Edwards
21. The Calvarial Injection Assay
Robert J. van 't Hof 22. In Vivo Models of Mechanical Loading Behzad Javaheri, Nathalie Bravenboer, Astrid D. Bakker, Albert van der Veen, Roberto Lopes de Souza, Leanne Saxon, and Andrew A. Pitsillides
23. Animal Models for the Study of Bone Derived Pain Austen L. Thompson, Tally M. Largent-Milnes, and Todd W. Vanderah
Part V: Microscopical and Imaging Techniques
24. Histomorphometry in Rodents
Reinhold G. Erben and Martin Glösmann
25. Immunostaining of Skeletal Tissues Anke J. Roelofs and Cosimo De Bari
26. Techniques for the Study of Apoptosis in Bone Val Mann and Brendon Noble
27. Live Cell Imaging of Bone Cell and Organ Cultures
Sarah L. Dallas, Patricia A. Veno, and LeAnn M. Tiede-Lewis 28. Analysis of Bone Architecture in Rodents using micro Computed Tomography Robert J. van 't Hof and Enrico Dall'Ara 29. Bone Measurements by Peripheral Quantitative Computed Tomography in Rodents
Jürg A. Gasser and Johannes Willnecker
30. Quantitative X-ray Imaging of Mouse Bone by Faxitron Natalie C Butterfield, John G Logan, Julian Waung, Graham R Williams, and JH Duncan Bassett
31. Scanning Electron Microscopy of Bone
Alan Boyde
32. Transmission Electron Microscopy of Bone
Vincent Everts, Anneke Niehof, Wikky Tigchelaar-Gutter, and Wouter Beertsen
33. Tracking Cancer Cells Colonisation in Rodent Bone Using Two-photon Microscopy
Gloria Allocca and Ning Wang
34. Fourier Transform Infrared Imaging of Bone
Eleftherios P. Paschalis
35. Raman Microscopy and Bone
Simon R. Goodyear and Richard M. Aspden
14. In Vivo Models of Rheumatoid Arthritis Bettina Grötsch, Aline Bozec and Georg Schett 15. Osteoarthritis Mouse Model of Destabilisation of the Medial Meniscus
Antonia Sophocleous and Carmen Huesa 16. Models of Prostate Cancer Bone Metastasis
Sun Hee Park, Matthew Robert Eber, andYusuke Shiozawa
17. Animal Models of Breast Cancer Bone Metastasis
Claudia Tulotta, Arwin Groenewoud, B. Ewa Snaar-Jagalska, and Penelope Ottewell
18. Murine Models of Bone Sarcomas
Camille Jacques, Nathalie Renema, Benjamin Ory,Carl R. Walkley, Agi E. Grigoriadis, and Dominique Heymann
19. Mouse Models of Melanoma Bone Metastasis
Özge Uluçkan
20. Animal Models of Multiple Myeloma
Sam W.Z. Olechnowicz and Claire M. Edwards
21. The Calvarial Injection Assay
Robert J. van 't Hof 22. In Vivo Models of Mechanical Loading Behzad Javaheri, Nathalie Bravenboer, Astrid D. Bakker, Albert van der Veen, Roberto Lopes de Souza, Leanne Saxon, and Andrew A. Pitsillides
23. Animal Models for the Study of Bone Derived Pain Austen L. Thompson, Tally M. Largent-Milnes, and Todd W. Vanderah
Part V: Microscopical and Imaging Techniques
24. Histomorphometry in Rodents
Reinhold G. Erben and Martin Glösmann
25. Immunostaining of Skeletal Tissues Anke J. Roelofs and Cosimo De Bari
26. Techniques for the Study of Apoptosis in Bone Val Mann and Brendon Noble
27. Live Cell Imaging of Bone Cell and Organ Cultures
Sarah L. Dallas, Patricia A. Veno, and LeAnn M. Tiede-Lewis 28. Analysis of Bone Architecture in Rodents using micro Computed Tomography Robert J. van 't Hof and Enrico Dall'Ara 29. Bone Measurements by Peripheral Quantitative Computed Tomography in Rodents
Jürg A. Gasser and Johannes Willnecker
30. Quantitative X-ray Imaging of Mouse Bone by Faxitron Natalie C Butterfield, John G Logan, Julian Waung, Graham R Williams, and JH Duncan Bassett
31. Scanning Electron Microscopy of Bone
Alan Boyde
32. Transmission Electron Microscopy of Bone
Vincent Everts, Anneke Niehof, Wikky Tigchelaar-Gutter, and Wouter Beertsen
33. Tracking Cancer Cells Colonisation in Rodent Bone Using Two-photon Microscopy
Gloria Allocca and Ning Wang
34. Fourier Transform Infrared Imaging of Bone
Eleftherios P. Paschalis
35. Raman Microscopy and Bone
Simon R. Goodyear and Richard M. Aspden
... weniger
Bibliographische Angaben
- 2019, 3. Aufl., XV, 670 Seiten, 121 farbige Abbildungen, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Aymen I. Idris
- Verlag: Springer, Berlin
- ISBN-10: 1493989960
- ISBN-13: 9781493989966
- Erscheinungsdatum: 08.02.2019
Sprache:
Englisch
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