Cancer Immunosurveillance

Usage of Droplet Digital PCR (ddPCR) Assays for T Cell Quantification in Cancer.- An Economical, Quantitative, and Robust Protocol for High Throughput T Cell Receptor Sequencing from Tumor or Blood.- Generation of Tumor Antigen-Specific Cytotoxic T Lymphocytes from Pluripotent Stem Cells.- Expansion and Adoptive Transfer of Human Vd2+ T Cells to Assess Anti-Tumor Effects In Vivo.- Usage of Single-Cell RNA-Sequencing to Unveil Immune Lymphoid Cell Precursors.- Isolation, Expansion, and Characterization of Natural Killer Cells and Their Precursors as a Tool to Study Cancer Immunosurveillance.- CD107a Degranulation Assay to Evaluate Immune Cell Anti-Tumor Activity.- A Flow Cytometric NK Cell-Mediated Cytotoxicity Assay to Evaluate Anti-Cancer Immune Responses In Vitro.- Mouse Models to Study Natural Killer Cell-Mediated Immunosurveillance and Metastatic Latency.- Tumor-Associated Macrophage Isolation and In Vivo Analysis of their Tumor Promoting Activity.- Conditional Genetic Ablation Mouse Models as a Tool to Study Cancer Immunosurveillance In Vivo.- Determination and Isolation of Immune Populations from Brain Tumor Microenvironments.- Mouse Model of Colitis Associated Colorectal Cancer (CAC): Isolation and Characterization of Mucosal-Associated Lymphoid Cells.- Cancer Exome-Based Identification of Tumor Neo-Antigens Using Mass Spectrometry.- Single-Cell Mass Cytometry of Archived Human Epithelial Tissue for Decoding Cancer Signaling Pathways.- Purification of Leukemia-Derived Exosomes to Study Microenvironment Modulation.- Molecular Characterization of Circulating Tumor Cells to Study Cancer Immunoevasion.- Quantitative Identification of Senescent Cells in Cancer.- Generation and Harnessing of Heterotypic Tumor-Stroma Spheroids to Study Cancer Immunosurveillance.- Establishment of Slice Cultures as a Tool to Study the Cancer Immune Microenvironment.- Gold