Carbohydrate-Based Vaccines
Methods and Protocols
(Sprache: Englisch)
This volume summarizes current cutting-edge methods related to carbohydrate-based vaccines, from the identification of a suitable carbohydrate antigen via the preparation of glycoconjugate vaccines to the characterization of vaccine candidates for their use...
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This volume summarizes current cutting-edge methods related to carbohydrate-based vaccines, from the identification of a suitable carbohydrate antigen via the preparation of glycoconjugate vaccines to the characterization of vaccine candidates for their use in pre-clinical and clinical studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Carbohydrate-Based Vaccines: Methods and Protocols aims to be a valuable manual that will facilitate research in the field of carbohydrate-based vaccines.
Inhaltsverzeichnis zu „Carbohydrate-Based Vaccines “
1. Carbohydrate-based Vaccines: An OverviewJulia Hütter and Bernd Lepenies
2. Glycans as Vaccine Antigens and Adjuvants: Immunological Considerations
Stephanie Zimmermann and Bernd Lepenies
3. The Glycan Array Platform as a Tool to Identify Carbohydrate Antigens
Li Xia and Jeffrey C. Gildersleeve
4. Antibody-carbohydrate Recognition from Docked Ensembles using
the AutoMap Procedure
Tamir Dingjan, Mark Agostino, Paul A. Ramsland, and Elizabeth Yuriev
5. Generation of Monoclonal Antibodies against Defined Oligosaccharide Antigens
Felix Broecker, Chakkumkal Anish, and Peter H. Seeberger
6. Murine Whole-Blood Opsonophagocytosis Assay to Evaluate Protection by Antibodies Raised Against Encapsulated Extracellular Bacteria
Guillaume Goyette-Desjardins, René Roy, and Mariela Segura
7. Determination of N-linked Glycosylation in Viral Glycoproteins by Negative Ion Mass Spectrometry and Ion Mobility
David Bitto, David J. Harvey, Steinar Halldorsson, Katie J. Doores, Laura K. Pritchard,
Juha T. Huiskonen,Thomas A. Bowden, and Max Crispin
8. N-Glycosylation Fingerprinting of Viral Glycoproteins by xCGE-LIF
René Hennig , Erdmann Rapp, Robert Kottler, Samanta Cajic , Matthias Borowiak , and Udo Reichl
9. Temporary Conversion of Protein Amino Groups To Azides: A Synthetic Strategy For Glycoconjugate Vaccines
Tomasz Lipinski and David R. Bundle
10. Gold Nanoparticles as Carriers For Synthetic Glycoconjugate Vaccines
Fabrizio Chiodo and Marco Marradi
11. Identification and Characterization of Carbohydrate-Based Adjuvants
Timo Johannssen and Bernd Lepenies
12. Characterization of Carbohydrate Vaccines by NMR Spectroscopy
Francesco Berti and Neil Ravenscroft
13. Characterisation of Capsular Polysaccharides and Their Glycoconjugates by Hydrodynamic Methods
Stephen E. Harding, Ali Saber Abdelhameed, Richard B. Gillis, Gordon A. Morris, and Gary G. Adams
14. Glycoconjugate Vaccines: The Regulatory
... mehr
Framework
Christopher Jones
Christopher Jones
... weniger
Bibliographische Angaben
- 2015, 1st ed. 2015, XI, 255 Seiten, 21 farbige Abbildungen, Maße: 18,5 x 26,2 cm, Gebunden, Englisch
- Herausgegeben: Bernd Lepenies
- Verlag: Springer, Berlin
- ISBN-10: 1493928732
- ISBN-13: 9781493928736
Sprache:
Englisch
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