Double-Stranded RNA

Isolation of Double-Stranded RNAs by Lithium Chloride Fractionation.- Detection of dsRNA by Acridine Orange Staining.- Isolation of dsRNA from Plants by Cellulose Chromatography.- Rapid Purification of dsRNA Using Micro-Spin Cellulose Column.- Analysis of Virus-Induced Double-Stranded RNA in Living Plant Cells by the dRBFC Assay.- Detection of dsRNA with the Fluorescence In Situ Hybridization (FISH) Assay.- Subcellular Colocalization Assay of Host Factors with Viral Replication Complex in the dsRNA Reporter Nicotiana benthamiana.- Production of Double-Stranded RNA Using the Prokaryotic Promoter-Mediated Bidirectional Transcription.- Inducible Expression of dsRNA in Escherichia coli.- In Vivo Production of dsRNA Using Bacteriophage 6 in Pseudomonas syringae Cit7 Cells.- Bacteria-Based Double-Stranded RNA Production to Develop Cost-Effective RNA Interference Application for Insect Pest Management.- Transiently Induce RNA Silencing in Plants Using a TobaccoNecrosis Virus A (TNV-A)-Based dsRNA Production System.- Co-Immunoprecipitation-Based Isolation of Double-Stranded RNA-Associated Protein Complexes in Nicotiana benthamiana.- Analysis of Plant Virus-Induced Immunity by Using Viral-Derived Double-Stranded RNA in Arabidopsis thaliana.- Identification of Mycoviruses by dsRNA Extraction.- Production of Double-Stranded RNA In Planta by a Potato Mop-Top Virus (PMTV)-Based Vector for Inducing Gene Silencing.- Application of dsRNA for Fungi Disease Management Sclerotinia sclerotiorum and Botrytis cinerea.- Application of dsRNA in the Pine Wood Nematode, Bursaphelenchus xylophilus.