Flow Cytometry Protocols

 Flow Cytometry: The Glass Is Half Full.- Basic Principles of Flow Cytometer Operation: The Make Your Own Flow Cytometer.- Lasers Sources for Traditional and Spectral Flow Cytometry.- How to Measure "Spillover Spread".- Comprehensive Immunophenotyping by Polychromatic Cytometry.- Panel Design and Optimization for Full Spectrum Flow Cytometry.- Practicalities of Cell Sorting.- Image-Enabled Cell Sorting Using the BD CellView Technology.- Monitoring Cell Proliferation by Dye Dilution: Considerations for Panel Design.- Multiparametric Analysis of Apoptosis by Flow Cytometry.- Quantitative and Standardized Pseudovirus Neutralization Assay for COVID-19.- CRISPR-Cas9-Mediated Bioluminescent Tagging of Endogenous Proteins by Fluorescent Protein-Assisted Cell Sorting.- Co-Staining with Fluorescent Antibodies and Antibody-Derived Tags for Cell Sorting Prior to CITE-seq.- Discovery of Live Cell Selective Fluorescent Probesand Elucidation of Their Mechanisms: Case Study of B Cell Selective Probe CDgB.- Fluorescence Lifetime Measurements and Analyses: Protocols Using Flow Cytometry and High-Throughput Microscopy.- Using Artificial Intelligence to Interpret Clinical Flow Cytometry Data Sets for Automated Disease Diagnosis and/or Monitoring.- Approaching Mass Cytometry Translational Studies by Experimental and Data Curation Settings.- CyTOF Intracellular Cytokine Assays for Antigen-Specific T Cells.- Imaging Mass Cytometry for In Situ Immune Profiling.- Cytometry in High-Containment Laboratories.