Genotyping
Methods and Protocols
(Sprache: Englisch)
This volume explores a broad range of different genotyping techniques. Genotyping: Methods and Protocols consists of chapters that cover numerous topics such as: an overview of multiplexed microsatellite analysis; High Resolution Melt analysis and...
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Klappentext zu „Genotyping “
This volume explores a broad range of different genotyping techniques. Genotyping: Methods and Protocols consists of chapters that cover numerous topics such as: an overview of multiplexed microsatellite analysis; High Resolution Melt analysis and TaqMan-based assays; in situ analysis of variants in single RNA molecules; the MassARRAY system and Molecular Inversion Probes; Pulsed Field Gel Electrophoresis, Paralogue Ratio Test, and Multiplex Ligation-Dependent Probe Amplification; long-range PCR combined with PacBio sequencing; Targeted Locus Amplification; Multilocus Sequence Typing and rapid SNP detection with pyrosequencing; and genotyping-by-sequencing for plant analysis. Finally, the volume concludes with a summary of pertinent points to describe genetic variation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and practical, Genotyping: Methods and Protocols is a valuable resource for anyone interested in learning more about the diverse field of genotyping.
Inhaltsverzeichnis zu „Genotyping “
1. Genetic Fingerprinting Using Microsatellite Markers in a Multiplex PCR Reaction: A Compilation of Methodological Approaches from Primer Design to Detection Systems Jacqueline Krüger and Dorit Schleinitz 2. Genotyping DNA Variants with High Resolution Melting Analysis Rolf H.A.M. Vossen 3. High-Throughput Genotyping with TaqMan Allelic Discrimination and Allele-Specific Genotyping Assays Angelika Heissl, Barbara Arbeithuber, and Irene Tiemann-Boege 4. In Situ Single Molecule RNA Genotyping Using Padlock Probes and Rolling Circle Amplification Tomasz Krzywkowski, Thomas Hauling, and Mats Nilsson 5. The MassARRAY® System for Targeted SNP Genotyping Justine A. Ellis and Benjamin Ong 6. Targeted Capture and High-Throughput Sequencing Using Molecular Inversion Probes (MIPs) Stuart Cantsilieris, Holly A. Stessman, Jay Shendure, and Evan E. Eichler 7. Analyzing Copy Number Variation Using Pulsed-Field Gel Electrophoresis: Providing a Genetic Diagnosis for FSHD Richard J.L.F. Lemmers 8. Analysis of Copy Number Variation Using the Paralogue Ratio Test (PRT) Edward J. Hollox 9. Genotyping Complex Copy Number Variation with Multiplex Ligation-Dependent Probe Amplification (MLPA) Suzan de Boer and Stefan J. White 10. Analysis of Multiallelic CNVs by Emulsion Haplotype Fusion PCR Jess Tyson and John A.L. Armour 11. Quantitative DNA Analysis Using Droplet Digital PCR Rolf H.A.M. Vossen and Stefan J. White 12. Full-Length Mitochondrial-DNA Sequencing on the PacBio RSII and Full-Length MT-DNA Sequencing on the PacBio RSII Rolf H.A.M. Vossen and Henk P.J. Buermans 13. Targeted Locus Amplification and Next Generation Sequencing Quint P. Hottentot, Max van Min, Erik Splinter, Stefan J. White 14. Efficient, Cost-Effective, High-Throughput, Multilocus Sequencing Typing (MLST) Method, NGMLST, and the Analytical Software Program MLSTEZ Yuan Chen and John R. Perfect 15. Rapid SNP Detection and Genotyping of Bacterial Pathogens by Pyrosequencing Kingsley K. Amoako, Matthew C. Thomas,
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Timothy W. Janzen, and Noriko Goji 16. Methods for Genotyping-by-Sequencing Beth A. Rowan, Danelle K. Seymour, Eunyoung Chae, Derek S. Lundberg, and Detlef Weigel 17. Describing Sequence Variants Using HGVS Nomenclature Johan T. den Dunnen
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Bibliographische Angaben
- 2016, 1st ed. 2017, X, 254 Seiten, 55 farbige Abbildungen, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Stefan J. White, Stuart Cantsilieris
- Verlag: Springer, Berlin
- ISBN-10: 1493964402
- ISBN-13: 9781493964406
- Erscheinungsdatum: 08.11.2016
Sprache:
Englisch
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