Human Pluripotent Stem Cells
Methods and Protocols
(Sprache: Englisch)
This authoritative book is as an ideal guide to scientists conducting their own pluripotent cell research programs. The book aims to assist those making strides in this field towards furthering human knowledge and, ultimately, improving the human condition.
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Produktinformationen zu „Human Pluripotent Stem Cells “
This authoritative book is as an ideal guide to scientists conducting their own pluripotent cell research programs. The book aims to assist those making strides in this field towards furthering human knowledge and, ultimately, improving the human condition.
Klappentext zu „Human Pluripotent Stem Cells “
Almost daily, new technologies are being presented that move the field of human pluripotent stem cell research towards a future that may yield highly-effective, personalized medical treatments. Three enabling technologies at hand for human PSCs are 1) directed reprogramming of somatic cells, which eliminate many of the ethical issues associated with the derivation and use of human PSCs, increase genetic diversity of the available human PSC lines, and give rise to better in vitro human disease models; 2) the discovery that a Rho-associated protein Kinase (ROCK) inhibitor allows for efficient single cell passaging and cryopreservation, increasing the efficiency and reliability of hPSC culture; and 3) defined, animal-component-free media, which lay the groundwork for simplified scale-up for therapeutic applications, differentiation protocols, and toxicology screens. The aforementioned technologies can be found in Human Pluripotent Stem Cells: Methods and Protocols, a compilation of 33 detailed protocols in six categories of PSC research that cover laboratory essentials and the derivation of new PSC lines, including induced PSC lines, as well as their growth, maintenance, characterization, genetic manipulation, and differentiation. Written in the successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and accessible, Human Pluripotent Stem Cells: Methods and Protocols serves as an ideal guide to scientists conducting their own pluripotent cell research programs and makes great strides towards furthering human knowledge and, ultimately, improving the human condition.
Inhaltsverzeichnis zu „Human Pluripotent Stem Cells “
Section I. Laboratory Essentials 1. The Stem Cell Laboratory: Design, Equipment, and Oversight - Robin L. Wesselschmidt and Philip H. Schwartz
2. Stem Cell Banks: Preserving Cell Lines, Maintaining Genetic Integrity, and Advancing Research - Lesley Young, Lyn Healy, and Glyn N. Stacey
Section II. Derivation
3. Embryonic Stem Cell Derivation from Human Embryos - Paul Lerou
4. Derivation of Human Parthenogenetic Stem Cell Lines - Nikolay Turovets, AndreY Semechkin, Leonid Kuzmichev, Jeffrey Janus, Larissa Agapova, and Elena Revazova
5. Generation of Induced Pluripotent Stem Cell Lines from Human Fibroblasts via Retroviral Gene Transfer - Justine D. Miller and Thorsten M. Schlaeger
6. Derivation of Induced Pluripotent Stem Cells by Lentiviral Transduction - Hubert E. Nethercott, David J. Brick, and Philip H. Schwartz
7. Transgene-free Production of Pluripotent Stem Cells using PiggyBac Transposons - Knut Woltjen, Riikka Hämäläinen, Mark Kibschull, Maria Mileikovsky, and Andras Nagy
Section III. Growth, Maintenance and Expansion
8. Traditional Human Embryonic Stem Cell Culture - Philip H. Schwartz, David J. Brick, Hubert E. Nethercott, and Alexander E. Stover
9. Xeno-Free Culture of Human Pluripotent Stem Cells - Rosita Bergström, Susanne Ström, Frida Holm, Anis Feki, and Outi Hovatta
10. Adaptation of Human Pluripotent Stem Cells to Feeder-Free Conditions in Chemically-Defined Medium with Enzymatic Single-Cell Passaging - Alexander E. Stover and Philip H. Schwartz
11. GMP Scale-Up and Banking of Pluripotent Stem Cells for Cellular Therapy Applications - Lara J. Ausubel, Patricia M. Lopez, and Larry A. Couture
12. Culture of Human Pluripotent Stem Cells on Glass Slides for High-Resolution Imaging - Victoria Fox
Section IV. Characterization
13. Classical Cytogenetics: Karyotyping Techniques - Steven E. Bates
14. FISH Analysis of Human Pluripotent Stem Cells - Suzanne
... mehr
E. Peterson, Jerold Chun, and Jeanne Loring
15. Immunocytochemical Analysis of Human Pluripotent Stem Cells - Hubert E. Nethercott, David J. Brick, and Philip H. Schwartz
16. Flow Cytometric Analysis of Human Pluripotent Stem Cells - Mirabelle SH. Ho, Andrew Fryga, and Andrew L. Laslett
17. The Teratoma Assay: An In vivo Assessment of Pluripotency - Robin L. Wesselschmidt
18. Detection of Copy Number Variation using SNP Genotyping - Gregory M. Cooper and Heather C. Mefford
19. Genome-wide Epigenetic Analysis of Human Pluripotent Stem Cells by ChIP and ChIP-Seq - Michael J. Hitchler and Judd C. Rice
20. Basic Approaches to Gene Expression Analysis of Stem Cells by Microarrays - Bernhard Schuldt, Qiong Lin, Franz-Josef Müller, and Jeanne F. Loring
21. Development of High Content Screening Approaches and Analysis for Human Pluripotent Stem Cells - Sean P. Sherman, Jackelyn A. Alva, Kaushali Thakore-Shah, and April D. Pyle
22. Quantitative Proteome and Phosphoproteome Analysis of Human Pluripotent Stem Cells - Javier Muñoz and Albert J. R. Heck
Section V. Genetic Manipulation (Gene Modulation)
23. Lentivirus-mediated Modification of Pluripotent Stem Cells - Ruchi Bajpai
24. Nucleofection of Human Embryonic Stem Cells - Helen Fong, K. A. Hohenstein Elliott, Leslie F. Lock, and Peter J. Donovan
25. Non-viral Gene Delivery in Neural Progenitors Derived from Human Pluripotent Stem Cells - Sujoy K. Dhara, Anirban Majumder, Mahesh C. Dodla, and Steven L. Stice
26. Gene Targeting in Human Pluripotent Stem Cells - Ying Liu and Mahendra Rao
27. Episomal Transgene Expression in Pluripotent Stem Cells - Michele M.P. Lufino, Anna R. Popplestone, Sally A. Cowley, Pauline A.H. Edser, William S. James, and Richard Wade-Martins
Section VI: Differentiation
28. The Generation of Embryoid Bodies from Feeder-based or Feeder-free Human Pluripotent Stem Cell Cultures - Alexander E. Stover and Philip H. Schwartz
29. Derivation of Oligodendrocyte Progenitor Cells from Human Embryonic Stem Cells - Jason Sharp, Maya Hatch, Gabriel Nistor, and Hans Keirstead
30. Directed Differentiation of Dopamine Neurons From Human Pluripotent Stem Cells - Lixiang Ma, Yan Liu, and Su-Chun Zhang
31. Methods for the Derivation and Use of Cardiomyocytes from Human Pluripotent Stem Cells - Wei-Zhong Zhu, Benjamin Van Biber, and Michael A. Laflamme
32. In Vivo Evaluation of Putative Hematopoietic Stem Cells Derived from Human Pluripotent Stem Cells - Melinda K. Hexum, Xinghui Tian, and Dan S. Kaufman
33. Differentiation of Dendritic Cells from Human Embryonic Stem Cells - Kathryn M. Silk, Su-Yi Tseng, Kevin P. Nishimoto, Jane S. Lebkowski, Anita Reddy, and Paul J. Fairchild
15. Immunocytochemical Analysis of Human Pluripotent Stem Cells - Hubert E. Nethercott, David J. Brick, and Philip H. Schwartz
16. Flow Cytometric Analysis of Human Pluripotent Stem Cells - Mirabelle SH. Ho, Andrew Fryga, and Andrew L. Laslett
17. The Teratoma Assay: An In vivo Assessment of Pluripotency - Robin L. Wesselschmidt
18. Detection of Copy Number Variation using SNP Genotyping - Gregory M. Cooper and Heather C. Mefford
19. Genome-wide Epigenetic Analysis of Human Pluripotent Stem Cells by ChIP and ChIP-Seq - Michael J. Hitchler and Judd C. Rice
20. Basic Approaches to Gene Expression Analysis of Stem Cells by Microarrays - Bernhard Schuldt, Qiong Lin, Franz-Josef Müller, and Jeanne F. Loring
21. Development of High Content Screening Approaches and Analysis for Human Pluripotent Stem Cells - Sean P. Sherman, Jackelyn A. Alva, Kaushali Thakore-Shah, and April D. Pyle
22. Quantitative Proteome and Phosphoproteome Analysis of Human Pluripotent Stem Cells - Javier Muñoz and Albert J. R. Heck
Section V. Genetic Manipulation (Gene Modulation)
23. Lentivirus-mediated Modification of Pluripotent Stem Cells - Ruchi Bajpai
24. Nucleofection of Human Embryonic Stem Cells - Helen Fong, K. A. Hohenstein Elliott, Leslie F. Lock, and Peter J. Donovan
25. Non-viral Gene Delivery in Neural Progenitors Derived from Human Pluripotent Stem Cells - Sujoy K. Dhara, Anirban Majumder, Mahesh C. Dodla, and Steven L. Stice
26. Gene Targeting in Human Pluripotent Stem Cells - Ying Liu and Mahendra Rao
27. Episomal Transgene Expression in Pluripotent Stem Cells - Michele M.P. Lufino, Anna R. Popplestone, Sally A. Cowley, Pauline A.H. Edser, William S. James, and Richard Wade-Martins
Section VI: Differentiation
28. The Generation of Embryoid Bodies from Feeder-based or Feeder-free Human Pluripotent Stem Cell Cultures - Alexander E. Stover and Philip H. Schwartz
29. Derivation of Oligodendrocyte Progenitor Cells from Human Embryonic Stem Cells - Jason Sharp, Maya Hatch, Gabriel Nistor, and Hans Keirstead
30. Directed Differentiation of Dopamine Neurons From Human Pluripotent Stem Cells - Lixiang Ma, Yan Liu, and Su-Chun Zhang
31. Methods for the Derivation and Use of Cardiomyocytes from Human Pluripotent Stem Cells - Wei-Zhong Zhu, Benjamin Van Biber, and Michael A. Laflamme
32. In Vivo Evaluation of Putative Hematopoietic Stem Cells Derived from Human Pluripotent Stem Cells - Melinda K. Hexum, Xinghui Tian, and Dan S. Kaufman
33. Differentiation of Dendritic Cells from Human Embryonic Stem Cells - Kathryn M. Silk, Su-Yi Tseng, Kevin P. Nishimoto, Jane S. Lebkowski, Anita Reddy, and Paul J. Fairchild
... weniger
Autoren-Porträt
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Bibliographische Angaben
- 2011, XX, 468 Seiten, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Philip H. Schwartz, Robin Wesselschmidt
- Verlag: Springer, Berlin
- ISBN-10: 1617792004
- ISBN-13: 9781617792007
- Erscheinungsdatum: 09.08.2011
Sprache:
Englisch
Rezension zu „Human Pluripotent Stem Cells “
From the reviews:"The volume is a compilation of 33 detailed protocols divided into six parts ... . All the protocols are superbly illustrated with many figures and charts presented in full colours. I think this book is a must for all those are working with human pluripotent stem cells, no matter if students entering the field or colleagues already involved, each of them will get the chance to sharpen their conceptual insights." (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 56, 2012)
Pressezitat
From the reviews:"The volume is a compilation of 33 detailed protocols divided into six parts ... . All the protocols are superbly illustrated with many figures and charts presented in full colours. I think this book is a must for all those are working with human pluripotent stem cells, no matter if students entering the field or colleagues already involved, each of them will get the chance to sharpen their conceptual insights." (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 56, 2012)
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