Intracellular Lipid Transport

1. Analysis of Phosphatidylinositol Transfer at ER-PM Junctions in Receptor-stimulated Live Cells.- 2. Monitoring non-vesicular transport of phosphatidylserine and phosphatidylinositol 4-phosphate in intact cells by BRET analysis.- 3. Development of non-specific BRET-based biosensors to monitor plasma membrane inositol lipids in living cells.- 4. Following anterograde transport of phosphatidylserine in yeast in real time.- 5. Imaging lipid metabolism at the Golgi complex.- 6. Advanced in vitro assay system to measure PS and PE transport at ER/mitochondria interface.- 7. Measurement of lipid transport in mitochondria by the MTL complex.- 8. Bi- and trifunctional lipids for visualization of sphingolipid dynamics within the cell.- 9. Indirect lipid transfer protein activity measurements using quantification of glycosphingolipid production.- 10. Measurement of intracellular sterol transport in yeast.- 11. Intracellular and plasma membrane cholesterol labeling and quantification using filipin and GFP-D4.- 12. Monitoring and modulating intracellular cholesterol trafficking using ALOD4, a cholesterol-binding protein.- 13. Measurement of Lysophospholipid Transport Across the Membrane Using Escherichia coli Spheroplasts.- 14. Preparation of proteoliposomes with purified TMEM16 protein for accurate measures of lipid scramblase activity.- 15. In vitro assays to measure the membrane tethering and lipid transport activities of the Extended Synaptotagmins.- 16. Determining the lipid binding specificity of SMP domains:  an ERMES subunit as a case study.- 17. In Vitro Measurement of Sphingolipid Intermembrane Transport Illustrated by GLTP Superfamily Members.- 18. Purification and Characterization of Human Niemann-Pick C1 Protein.- 19. In vitro strategy to measure sterol/phosphatidylinositol 4-phosphate exchange between membranes.- 20. Determination of Ligand Binding Affinity