Jasmonate Signaling
Methods and Protocols
(Sprache: Englisch)
This book examines physiological, environmental, molecular, omics, and bioinformatics approaches that allow dissecting jasmonate actions in the model species Arabidopsis thaliana or in other plants. It features readily reproducible laboratory protocols.
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Produktinformationen zu „Jasmonate Signaling “
This book examines physiological, environmental, molecular, omics, and bioinformatics approaches that allow dissecting jasmonate actions in the model species Arabidopsis thaliana or in other plants. It features readily reproducible laboratory protocols.
Klappentext zu „Jasmonate Signaling “
It is now well established that jasmonates, originally identified as the major component of jasmine scent, play a universal role in the plant kingdom and are involved in the regulation of diverse aspects of plant biology, including growth, development, metabolism, and interaction with the environment. In Jasmonate Signaling: Methods and Protocols, experts in the field aim to unite powerful emerging omics platforms with a number of key reductionist approaches to form a comprehensive collection of tools and protocols. The detailed chapters in this book embrace physiological, environmental, molecular, omics, and bioinformatics approaches that allow dissecting jasmonate actions in the model species Arabidopsis thaliana or in other plants. Written in the highly successful Methods in Molecular Biology series format, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, along with tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Jasmonate Signaling: Methods and Protocols will empower interested researchers to dissect all steps of jasmonate signaling and the processes they modulate.
Inhaltsverzeichnis zu „Jasmonate Signaling “
Part I: Physiology1. Phenotyping Jasmonate Regulation of Senescence
Martin A. Seltmann and Susanne Berger
2. Characterizing Jasmonate Regulation of Male Fertility in Arabidopsis
Bryan Thines, Ajin Mandaokar, and John Browse
3. Phenotyping Jasmonate Regulation of Root Growth
Fabian Kellermeier and Anna Amtmann
Part II: Environment
4. Bioassays for Assessing Jasmonate-Dependent Defenses Triggered by Pathogens, Herbivorous Insects, or Beneficial Rhizobacteria
Saskia C.M. Van Wees, Johan A. Van Pelt, Peter A.H.M. Bakker, and Corné M.J. Pieterse
5. Elicitation of Jasmonate-Mediated Defense Responses by Mechanical Wounding and Insect Herbivory
Marco Herde, Abraham J.K. Koo, and Gregg A. Howe
6. Pseudomonas syringae Infection Assays in Arabidopsis
Jian Yao, John Withers, and Sheng Yang He
7. Jasmonate Signaling in the Field, Part I: Elicited Changes in Jasmonate Pools of Transgenic Nicotiana attenuata Populations
Emmanuel Gaquerel, Michael Stitz, Mario Kallenbach, and Ian T. Baldwin
8. Jasmonate Signaling in the Field, Part II: Insect-Guided Characterization of Genetic Variations in Jasmonate-Dependent Defenses of Transgenic and Natural Nicotiana attenuata
... mehr
Populations
Emmanuel Gaquerel, Michael Stitz, Mario Kallenbach, and Ian T. Baldwin
Part III: Molecules
9. Profiling of Jasmonic Acid-Related Metabolites and Hormones in Wounded Leaves
Yusuke Jikumaru, Mitsunori Seo, Hideyuki Matsuura, and Yuji Kamiya
10. A Non-Targeted Approach for Extended Liquid Chromatography-Mass Spectrometry Profiling of Free and Esterified Jasmonates After Wounding
Gaëtan Glauser and Jean-Luc Wolfender
11. Cell-Specific Detection of Jasmonates by Means of an Immunocytological Approach
Bettina Hause, Kati Mielke, and Susanne Forner
12. Jasmonic Acid-Amino Acid Conjugation Enzyme Assays
Martha L. Rowe and Paul E. Staswick
13. Pull-Down Analysis of Interactions Among Jasmonic Acid Core Signaling Proteins
Sandra Fonseca and Roberto Solano
14. Yeast Two-Hybrid Analysis of Jasmonate Signaling Proteins
Amparo Cuéllar Pérez, Laurens Pauwels, Rebecca de Clercq, and Alain Goossens
15. Modified Bimolecular Fluorescence Complementation Assay to Study the Inhibition of Transcription Complex Formation by JAZ Proteins
Tiancong Qi, Susheng Song, and Daoxin Xie
16. Agroinfiltration of Nicotiana benthamiana Leaves for Co-Localization of Regulatory Proteins Involved in Jasmonate Signaling
Volkan Çevik and Kemal Kazan
17. Electrophoretic Mobility Shift Assay for the Analysis of Interactions of Jasmonic Acid-Responsive Transcription Factors with DNA
Johan Memelink
18. Transient Expression Assays in Tobacco Protoplasts
Robin Vanden Bossche, Brecht Demedts, Rudy Vanderhaeghen, and Alain Goossens
19. Functional Analysis of Jasmonic Acid-Responsive Secondary Metabolite Transporters
Nobukazu Shitan, Akifumi Sugiyama, and Kazufumi Yazaki
20. Expression Analysis of Jasmonate-Responsive Lectins in Plants
Nausicaä Lannoo and Els J.M. Van Damme
Part IV: Omics
21. Profiling the Jasmonic Acid Responses by Nuclear Magnetic Resonance-Based Metabolomics
Hye Kyong Kim, Young Hae Choi, and Robert Verpoorte
22. Metabolite Profiling of Plant Tissues by Liquid Chromatography Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
Jacob Pollier and Alain Goossens
23. cDNA-AFLP-Based Transcript Profiling for Genome-Wide Expression Analysis of Jasmonate-Treated Plants and Plant Cultures
Janine Colling, Jacob Pollier, Nokwanda P. Makunga, and Alain Goossens
24. Analysis of RNA-Seq Data with TopHat and Cufflinks for Genome-Wide Expression Analysis of Jasmonate-Treated Plants and Plant Cultures
Jacob Pollier, Stephane Rombauts, and Alain Goossens
25. Transcriptome Coexpression Analysis Using ATTED-II for Integrated Transcriptomics/Metabolomics Analysis
Keiko Yonekura-Sakakibara and Kazuki Saito
26. A Guide to CORNET for the Construction of Coexpression and Protein-Protein Interaction Networks
Stefanie De Bodt and Dirk Inzé
Emmanuel Gaquerel, Michael Stitz, Mario Kallenbach, and Ian T. Baldwin
Part III: Molecules
9. Profiling of Jasmonic Acid-Related Metabolites and Hormones in Wounded Leaves
Yusuke Jikumaru, Mitsunori Seo, Hideyuki Matsuura, and Yuji Kamiya
10. A Non-Targeted Approach for Extended Liquid Chromatography-Mass Spectrometry Profiling of Free and Esterified Jasmonates After Wounding
Gaëtan Glauser and Jean-Luc Wolfender
11. Cell-Specific Detection of Jasmonates by Means of an Immunocytological Approach
Bettina Hause, Kati Mielke, and Susanne Forner
12. Jasmonic Acid-Amino Acid Conjugation Enzyme Assays
Martha L. Rowe and Paul E. Staswick
13. Pull-Down Analysis of Interactions Among Jasmonic Acid Core Signaling Proteins
Sandra Fonseca and Roberto Solano
14. Yeast Two-Hybrid Analysis of Jasmonate Signaling Proteins
Amparo Cuéllar Pérez, Laurens Pauwels, Rebecca de Clercq, and Alain Goossens
15. Modified Bimolecular Fluorescence Complementation Assay to Study the Inhibition of Transcription Complex Formation by JAZ Proteins
Tiancong Qi, Susheng Song, and Daoxin Xie
16. Agroinfiltration of Nicotiana benthamiana Leaves for Co-Localization of Regulatory Proteins Involved in Jasmonate Signaling
Volkan Çevik and Kemal Kazan
17. Electrophoretic Mobility Shift Assay for the Analysis of Interactions of Jasmonic Acid-Responsive Transcription Factors with DNA
Johan Memelink
18. Transient Expression Assays in Tobacco Protoplasts
Robin Vanden Bossche, Brecht Demedts, Rudy Vanderhaeghen, and Alain Goossens
19. Functional Analysis of Jasmonic Acid-Responsive Secondary Metabolite Transporters
Nobukazu Shitan, Akifumi Sugiyama, and Kazufumi Yazaki
20. Expression Analysis of Jasmonate-Responsive Lectins in Plants
Nausicaä Lannoo and Els J.M. Van Damme
Part IV: Omics
21. Profiling the Jasmonic Acid Responses by Nuclear Magnetic Resonance-Based Metabolomics
Hye Kyong Kim, Young Hae Choi, and Robert Verpoorte
22. Metabolite Profiling of Plant Tissues by Liquid Chromatography Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
Jacob Pollier and Alain Goossens
23. cDNA-AFLP-Based Transcript Profiling for Genome-Wide Expression Analysis of Jasmonate-Treated Plants and Plant Cultures
Janine Colling, Jacob Pollier, Nokwanda P. Makunga, and Alain Goossens
24. Analysis of RNA-Seq Data with TopHat and Cufflinks for Genome-Wide Expression Analysis of Jasmonate-Treated Plants and Plant Cultures
Jacob Pollier, Stephane Rombauts, and Alain Goossens
25. Transcriptome Coexpression Analysis Using ATTED-II for Integrated Transcriptomics/Metabolomics Analysis
Keiko Yonekura-Sakakibara and Kazuki Saito
26. A Guide to CORNET for the Construction of Coexpression and Protein-Protein Interaction Networks
Stefanie De Bodt and Dirk Inzé
... weniger
Bibliographische Angaben
- 2013, 2013, XIII, 347 Seiten, 33 farbige Abbildungen, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Alain Goossens, Laurens Pauwels
- Verlag: Springer, Berlin
- ISBN-10: 1627034137
- ISBN-13: 9781627034135
- Erscheinungsdatum: 25.04.2013
Sprache:
Englisch
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