Lipopolysaccharide Transport

Use of Mutagenesis and Functional Screens to Characterize Essential Genes Involved in Lipopolysaccharide Transport.- Generation of Stable and Unmarked Conditional Mutants in Pseudomonas aeruginosa.- Analysing the Function of Essential Genes by Plasmid Shuffling.- Membrane Fractionation by Isopycnic Sucrose Density Gradient Centrifugation for Qualitative Analysis of LPS in Escherichia Coli.- Exploring The Topology of Cytoplasmic Membrane Proteins Involved in Lipopolysaccharide Biosynthesis by in silico and Biochemical Analyses.- Use of Site-Directed Spin Labeling EPR Spectroscopy to Study Protein-LPS Interactions.- A Photo-Crosslinking Approach to Monitor Assembly of an LptD Intermediate with LptE in a Living Cell.- Mass Spectrometry Analysis of Dynamics and Interactions of the LPS translocon LptDE.- Affinity Purification and Co-Immunoprecipitation of Trans-envelope Protein Complexes in Gram-negative Bacteria.- A Bacterial Two-Hybrid System for in vivo assays of Protein-Protein Interactions and Drug Discovery.- Detecting Lipoproteins Sneaking out of the Lipopolysaccharide Leaflet.- Dissecting Lipopolysaccharide Composition and Structure by GC-MS and MALDI Spectrometry.- Probing the Functional Interaction Interface of Lipopolysaccharide and Antimicrobial Peptides: A Solution-State NMR Perspective.- Cryo-EM Analysis of the Lipopolysaccharide Flippase MsbA.- Protein Crystallization of Two Recombinant Lpt Proteins.- Metabolic Incorporation of Azido-Sugars into LPS to Enable Live Cell Fluorescence Imaging.- Use of Atomic Force Microscopy to Characterize LPS Perturbations.