Lysosomes

SILAC-Based Comparative Proteomic Analysis of Lysosomes from Mammalian Cells Using LC-MS/MS.- Quantitative Profiling of Lysosomal Lipidome by Shotgun Lipidomics Analysis of N- And O-Glycosylation of Lysosomal Glycoproteins.- Analyzing Lysosome Related Organelles By Electron Microscopy.- Microscopic Analysis of Lysosomal Membrane Permeabilization.- Quantitative Co-localization and Pattern Analysis Of Endo-Lysosomal Cargo in Subcellular Image Cytometry and Validation on Synthetic Image Sets.- Preparation of a Two-Photon Fluorescent Probe for Imaging H2O2 in Lysosomes in Living Cells and Tissues.- Lysophagy: A Method for Monitoring Lysosomal Rupture Followed by Autophagy Dependent Recovery.- Delivery of Cargo to Lysosomes using Gneosomes.- Lysosomal Acidification in Cultured Astrocytes Using Nanoparticles.- Analysis of Lysosomal pH by Flow Cytometry Using FITC-dextran Loaded Cells.- Detection of Lysosomal Exocytosis in Platelets by Flow Cytometry.- Detection of Lysosomal Exocytosis by Surface Exposure of Lamp1 Luminal Epitopes.- Using the MEROPS Database for Investigation of Lysosomal Peptidases, their Inhibitors and Substrates.- Next Generation Sequencing Approaches to Define the Role of the Autophagy Lysosomal Pathway in Human Disease: The Example of Lysoplex.- Gelatin Zymography using Leupeptin for the Detection of Various Cathepsin L Forms.- Methods for Determination of -Glycosidase, -Glycosidase, and -Galactosidase Activities in Dried Blood Spot Samples.- Prenatal Diagnosis of Lysosomal Storage Disorders Using Chorionic Villi.- Lysosomal Biology in Cancer.