Metabolic Reprogramming

In Vivo Tissue Lipid Uptake in Antisense Oligonucleotides (ASO) Treated Mice.-  In Vivo Hepatic Triglyceride Secretion Rate in Antisense Oligonucleotides (ASO) Treated Mice.- Measurement of Fatty Acid Oxidation by High Resolution Respirometry: Special Considerations for Analysis of Skeletal and Cardiac Muscle and Adipose Tissue.- In Vivo Imaging of Bone Marrow Long Chain Fatty Acid Uptake.- Quantification and Tracing of Stable Isotope into Cysteine and Glutathione.- Targeted Quantification of Amino Acids by Dansylation.- Isolation of Mitochondria from Mouse Tissues for Functional Analysis.-Methods for Monitoring Mitochondrial Biogenesis and Turnover in Cultured Hepatocytes and Mouse Liver Using MitoTimer Reporter Assay.- Quantification of Intracellular ATP Content in Ex-Vivo GC B Cells.-UHPLC-HRMS-based Analysis of S-Hydroxymethyl-Glutathione, GSH and GSSG in Human Cells.- Quantitation of glutathione and oxidized glutathione ratios from biological matrices using LC-MS/MS.- Real-Time Monitoring of Hydrogen Peroxide Levels in Yeast and Mammalian Cells.- Tracer-Based Metabolic Analysis by NMR in Intact Perfused Human Liver Tissue.- 13C isotope labeling and mass spectrometric isotope enrichment analysis in acute brain slices.- Metabolite analyses using Nuclear Magnetic Resonance (NMR) spectroscopy in plasma of patients with prostate cancer.- Screening Kinase-Dependent Phosphorylation of Key Metabolic Reprogramming Regulators.- Measuring the Oxidation State and Enzymatic Activity of Glyceraldehyde Phosphate Dehydrogenase (GAPDH).-  Quantitation of Glutamine Synthetase-1 Activity in Drosophila Melanogaster.- Fixed Cell Immunofluorescence for Quantification of Hypoxia-Induced Changes in Histone Methylation.- Metabolic Reprogramming During B Cell Differentiation.- An Integrated Methodology to Quantify the Glycolytic Stress in Plasma Cell Myeloma in Response to Cytotoxic