Mitochondrial DNA

Isolation of functional mitochondria and pure mtDNA from murine tissues.- Isolating mitochondria, mitoplasts, and mtDNA from cultured mammalian cells.- Coupling differential centrifugation with exonuclease treatment and size exclusion       chromatography (DIFSEC) for purification of mtDNA from mammalian cells.- Isolation and Quality Control of Yeast Mitochondria.- Mitochondrial DNA isolation from plants.- Visualize the Distribution and Dynamics of Mitochondrial DNA (mtDNA) nucleoids with        Multiple Labelling Strategies.- Visualization of mtDNA using FISH.- In situ analysis of mitochondrial DNA synthesis using metabolic labeling coupled to     fluorescence microscopy.- Measurement of nucleoid size using STED microscopy.- How to quantify DNA compaction by TFAM with Acoustic Force Spectroscopy and Total          Internal Reflection Fluorescence microscopy.- Assessing TFAM binding to human mitochondrial DNA.- Identification of proximity interactors of mammalian nucleoid proteins by BioID.- Localization of mitochondrial nucleoids by transmission electron microscopy using the transgenic expression of the mitochondrial helicase TWINKLE and APEX2.- In vitro assays of TWINKLE function.- Rolling Circle Replication and Bypass of Damaged Nucleotides.- Studying mitochondrial nucleic acid synthesis utilizing intact isolated mitochondria.- Functional assessment of mitochondrial DNA maintenance by depletion and repopulation     using 2',3'-dideoxycytidine in cultured cells.- Analysis of mitochondrial DNA replication by two-dimensional agarose gel electrophoresis.- Quantitative analysis of nucleoside triphosphate pools in mouse muscle using hydrophilic        interaction liquid chromatography coupled with tandem mass spectrometry