Neutrophil Methods and Protocols

Neutrophils: An Overview.- The Role of Neutrophils in the Immune System.- Neutrophil Isolation and Subcellular Fractionation.- Isolation of Human Neutrophils From Venous Blood.- Neutrophil Isolation From Nonhuman Species.- Subcellular Fractionation of Human Neutrophils and Analysis of Subcellular Markers.- Biochemistry, Biology, and Signal Transduction of Neutrophils.- Rac and Rap GTPase Activation Assays.- Measurement of Phospholipid Metabolism in Intact Neutrophils.- Analysis of Protein Phosphorylation in Human Neutrophils.- Mitogen-Activated Protein Kinase Assays.- Transduction of Proteins into Intact Neutrophils.- Optical Methods for the Measurement and Manipulation of Cytosolic Free Calcium in Neutrophils.- Analysis of Electrophysiological Properties and Responses of Neutrophils.- Analysis of Neutrophil Apoptosis.- Neutrophil Adhesion and Chemotaxis.- Optical and Fluorescence Detection of Neutrophil Integrin Activation.- Analysis of Neutrophil Polarization and Chemotaxis.- Quantifying and Localizing Actin-Free Barbed Ends in Neutrophils.- Measurement of Neutrophil Adhesion Under Conditions Mimicking Blood Flow.- Model Systems to Investigate Neutrophil Adhesion and Chemotaxis.- Neutrophil Phagocytosis and Bactericidal Activity.- Immunofluorescence and Confocal Microscopy of Neutrophils.- Assessment of Phagosome Formation and Maturation by Fluorescence Microscopy.- Analysis of Neutrophil Membrane Traffic During Phagocytosis.- Analysis of Neutrophil Bactericidal Activity.- A Skin Chamber Technique as a Human Model for Studies of Aseptic Inflammatory Reactions.- Nadph Oxidase and Production of Reactive Oxygen Species.- Measurement of Respiratory Burst Products Generated by Professional Phagocytes.- The K-562 Cell Model for Analysis of Neutrophil NADPH Oxidase Function.- Cell-Free Assays.- Immunoaffinity Purification of Human Phagocyte Flavocytochrome b and Analysis of Conformational Dynamics.- Analysis of Neutrophil Gene Expression and Transcription Factors.-

From the reviews:

"...a carefully compiled collection of protocols that allow for study of many aspects of the neutrophil." -Doody's Book Review, Weighted Numerical Score:85-3 Stars

"This excellent book is one of the latest in the 'Methods in Molecular Biology' series ... . it comes beautifully presented and bound in a hardback cover and would grace any bookshelf. I would urge all self-respecting laboratories working with neutrophils - whether in research or diagnostics - to purchase one immediately. ... I was impressed by this book's comprehensive provision of protocols, addressing all aspects of neutrophil function and physiology, and including an excellent section on diagnosis of both inherited and non-inherited neutrophil disorders." (Lynn Morgan, Immunology News, May, 2008)