Osteoporosis
Methods and Protocols
(Sprache: Englisch)
In recent years, the study of bone cells and tissues at the cellular and molecular levels in a variety of models has revolutionized the field. In Osteoporosis: Methods and Protocols, leading scientists from around the world share their step-by-step...
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Klappentext zu „Osteoporosis “
In recent years, the study of bone cells and tissues at the cellular and molecular levels in a variety of models has revolutionized the field. In Osteoporosis: Methods and Protocols, leading scientists from around the world share their step-by-step laboratory protocols for studying bone biology. The topics covered in this volume include in vitro models, in vivo models utilized for drug testing, tissue engineering and osteoporosis studies in either gender, state-of-the-art molecular techniques to assess single genes or for global genomic analysis, powerful imaging techniques, and many more. As a volume in the highly successful Methods in Molecular Biology(TM) series, each chapter provides a brief introduction, a list of necessary materials, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Osteoporosis: Methods and Protocols is the perfect guide for both new and experienced experimentalists attempting to study the devastating silent disease known as osteoporosis.
Inhaltsverzeichnis zu „Osteoporosis “
- In vitro models1. Isolation and Culture of Rodent Osteoprogenitor Cells
Thomas A. Owen* and Lydia C. Pan
2. Osteoclast Culture and Resorption Assays
Elizabeth W. Bradley and Merry Jo Oursler*
3. Assessing New Bone Formation in Neonatal Calvarial Organ Cultures
Khalid S. Mohammad, John M. Chirgwin and Theresa Guise*
4. Detection of Apoptosis of Bone Cells In Vitro
Teresita Bellido*and Lilian I. Plotkin
In vivo rodent models
5. In Vivo Parathyroid Hormone Treatments and mRNA Isolation and Analysis
Xin Li, Ling Qin and Nicola C. Partridge*
6. Assessment of Bone Formation Capacity Using In Vivo Transplantation Assays: Procedure and Tissue Analysis
Badem M. Abdalleh, Nicholas Ditzel and Moustapha Kassem*
7. Ossicle and Vossicle Implant Model Systems
Glenda J. Pettway and Laurie K. McCauley*
8. Utility of the Ovariectomized (OVX) Rat as a Model for Human Osteoporosis In Drug Discovery
Yogendra P. Kharode*, Michael C. Sharp and Peter V. N. Bodine
9. Orchidectomy (ORX) Models of Osteoporosis
Stéphane Blouin, Hélène Libouban, Marie Françoise Moreau and Daniel Chappard*
Methods of gene delivery
10. Gene Delivery by Adenoviruses
Renny Franceschi* and Chunxi Ge
11. Lentiviral Delivery of shRNA Constructs into Osteoblasts
Marc N. Wein, Dallas C. Jones and Laurie H. Glimcher*
12. Gene Delivery by Retroviruses
Valerie Deregowski and Ernesto Canalis*
Molecular and GeneticTechniques
13. Chromatin Immunoprecipitation (ChIP) Assays: Application of ChIP-on-chip for Defining Dynamic Transcriptional Mechanisms in Bone Cells
Margaret van der Deen, Mohammad Q. Hassan, Jitesh Pratap, Nadiya M. Teplyuk, Daniel W. Young, Amjad Javed, Sayyed K. Zaidi, Jane B. Lian, Martin Montecino, Janet L. Stein, Gary S. Stein and Andre J. van Wijnen*
14. Identification of Transcription Factor Target Genes by ChIP Display (CD)
Artem Barski, Steve Pregizer and Baruch Frenkel*
15. Application of Laser Capture Microdissection (LCM)
... mehr
Technique for Molecular Definition of Skeletal Cell Differentiation In Vivo
Dafna Benayahu*, Rina Socher, and Irena Shur
16. Quantitative Trait Loci (QTL) Mapping
Dong-Hai Xiong, Jian-Feng Liu, Yan-Fang Guo, Yan Guo, Tie-Lin Yang, Shu-Gui Chen, Hui Jiang, Yuan Chen, Fang Yang, Robert R. Recker and Hong-Wen Deng*
Imaging Techniques
17. In Situ Nuclear Organization of Regulator Machinery
Shirwin M. Pockwinse, Sayyed K. Zaidi, Ricardo F. Medina, Rachit Bakshi, Krishna P. Kota, Syed A. Ali, Daniel W. Young, Jeffery A. Nickerson, Amjad Javed, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein and Gary S. Stein*
18. Bioluminescent Imaging in Bone
Yoram Zilberman, Yossi Gafni, Gadi Pelled, Zulma Gazit, and Dan Gazit*
19. Micro Computed Tomography - A Method for the Nondestructive Evaluation of the Three-Dimensional Structure of Biological Specimens
Martin Stauber and Ralph Müller*
20. Fourier Transform Infrared (FT-IR) Microspectroscopy and Microscopic Imaging
Samuel Gourion-Arsiquaud, Paul A. West and Adele L. Boskey*
Mechanical
Dafna Benayahu*, Rina Socher, and Irena Shur
16. Quantitative Trait Loci (QTL) Mapping
Dong-Hai Xiong, Jian-Feng Liu, Yan-Fang Guo, Yan Guo, Tie-Lin Yang, Shu-Gui Chen, Hui Jiang, Yuan Chen, Fang Yang, Robert R. Recker and Hong-Wen Deng*
Imaging Techniques
17. In Situ Nuclear Organization of Regulator Machinery
Shirwin M. Pockwinse, Sayyed K. Zaidi, Ricardo F. Medina, Rachit Bakshi, Krishna P. Kota, Syed A. Ali, Daniel W. Young, Jeffery A. Nickerson, Amjad Javed, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein and Gary S. Stein*
18. Bioluminescent Imaging in Bone
Yoram Zilberman, Yossi Gafni, Gadi Pelled, Zulma Gazit, and Dan Gazit*
19. Micro Computed Tomography - A Method for the Nondestructive Evaluation of the Three-Dimensional Structure of Biological Specimens
Martin Stauber and Ralph Müller*
20. Fourier Transform Infrared (FT-IR) Microspectroscopy and Microscopic Imaging
Samuel Gourion-Arsiquaud, Paul A. West and Adele L. Boskey*
Mechanical
... weniger
Bibliographische Angaben
- 2014, 2008, XIX, 354 Seiten, Maße: 15,5 x 23,5 cm, Kartoniert (TB), Englisch
- Herausgegeben: Jennifer Westendorf
- Verlag: Springer, Berlin
- ISBN-10: 1627038477
- ISBN-13: 9781627038478
Sprache:
Englisch
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