Phospholipid Signaling Protocols

Monitoring of Activation of Phospholipid-Derived Cell Signaling Pathways.- Phosphoinositidase C Activation Assay I.- Phosphoinositidase C Activation Assay II.- Phosphoinositidase C Activation Assay III.- Measurement of Phosphoinositols and Phosphoinositides Using Radio High-Performance Liquid Chromatography Flow Detection.- Preparation of [3H]Phosphoinositol Standards and Conversion of [3H]Phosphoinositides to [3H]Phosphoinositols.- Inositol 1,4,5-Trisphosphate Mass Assay.- Measurement of Cellular Diacylglycerol Content.- Phosphatidylinositol 4-Kinases.- Phosphatidylinositol(3,4,5)Trisphosphate (Ptdins(3,4,5)P3) Mass Measurement Using a Radioligand Displacement Assay.- Detection of Phosphatidylinositol-4-Phosphate 5-Kinase Activity Using Thin-Layer Chromatography.- Determination of Phospholipase C-or Phospholipase D-Catalyzed Phosphatidylcholine Hydrolysis.- Measurement of Phospholipase D Activity.- Monitoring of Phospholipase A2 Activation in Cultured Cells Using Tritiated Arachidonic Acid.- Assay of Cellular Diacylglycerol and Monoacylglycerol Lipases.- Isotopic Efflux Studies as Indices of Phospholipase Activation.- Extraction and Measurements of Prostanoids and Leukotrienes by Radioimmunoassays.- Measurements of Prostanoids, Leukotrienes, and Isoprostanes by Enzyme Immunoassays.- Measurements of Arachidonic Acid Metabolites Derived from the Lipoxygenase Pathways by High-Pressure Liquid Chromatography.- Measurement of Sphingomyelin and Ceramide Cellular Levels After Sphingomyelinase-Mediated Sphingomyelin Hydrolysis.- Sphingomyelin and Ceramide Mass Assay.- Sphingosine Kinase.- Analytical Methods and Steps to Sample Preparation for Determination of Molecular Species of Fatty Acids.- HPLC Analytical Methods for the Separation of Molecular Species of Fatty Acids in Diacylglycerol and Cellular Phospholipids.- Analysis of Molecular Species of Cellular Sphingomyelins and Ceramides.- General Methods for Monitoring Changes in Levels of Key Signaling Pathway Proteins and

". . . the detailed information provided in Phospholipid Signaling Protocols makes it a welcome addition to an academic library that serves scientists actively working in the area of second messenger and membrane lipid chemistry. It is well representative of the previous volumes in the Methods in Molecular Biology series, and therefore is a worthwhile acquisition."-E-Streams

". . .this book is an extremely valuable resource for methodology in studying the molecular species of key phospholipid signaling molecules, as well as the enzymes that produce or degrade them. I personally have used several protocols in this book, and have not had any trouble adapting the methods to my own applications."-The Quarterly Review of Biology

"...gives an excellent overview of the most common techniques in use as well in biochemistry and molecular biology...The editor is to be congratulated for putting these diverse, very important protocols together..."-Cell Biology International 2000

"...does a very good job of covering what is a fairly diverse field and the protocols described would allow any well equipped laboratory to tackle lipid analysis. There are currently very few books on the market addressing this important area of biochemistry and this volume would be a valuable addition to the collection of any laboratory studying lipid signaling molecules."-Molecular Biotechnology

"...does a very good job covering what is a fairly diverse field and the protocols described would allow any well equipped laboratory to tackle lipid analysis. There are currently very few books on the market addressing this important area of biochemistry and this volume would be a valuable addition to the collection of any laboratory studying lipid signalling molecules." - Molecular Biotechnology