Plant Functional Genomics

Finding Genes in Complex Plant Systems.- An Improved Method for Plant BAC Library Construction.- Constructing Gene-Enriched Plant Genomic Libraries Using Methylation Filtration Technology.- RescueMu Protocols for Maize Functional Genomics.- Precious Cells Contain Precious Information.- Combined ESTs from Plant-Microbe Interactions.- In Silico Prediction of Plant Gene Function.- Computer Software to Find Genes in Plant Genomic DNA.- Genomic Colinearity as a Tool for Plant Gene Isolation.- Using Natural Allelic Diversity to Evaluate Gene Function.- Quantitative Trait Locus Analysis as a Gene Discovery Tool.- Forward and Reverse Genetic Strategies.- Transposon Tagging Using Activator (Ac) in Maize.- T-DNA Mutagenesis in Arabidopsis.- Physical and Chemical Mutagenesis.- High-Throughput TILLING for Functional Genomics.- Gene and Enhancer Traps for Gene Discovery.- High-Throughput TAIL-PCR as a Tool to Identify DNA Flanking Insertions.- Custom Knock-Outs with Hairpin RNA-Mediated Gene Silencing.- Virus-Induced Gene Silencing.- Exploring the Potential of Plant RNase P as a Functional Genomics Tool.- Maintaining Collections of Mutants for Plant Functional Genomics.- Gain of Function Approaches.- Vector Construction for Gene Overexpression as a Tool to Elucidate Gene Function.- T-DNA Activation Tagging.- Phenotypic Profiling as a Tool to Determine Gene Function.- Expression Profiling Using cDNA Microarrays.- Open Architecture Expression Profiling of Plant Transcriptomes and Gene Discovery Using GeneCalling® Technology.- Proteomics as a Functional Genomics Tool.- Metabolite Profiling as a Functional Genomics Tool.- Growth Stage-Based Phenotypic Profiling of Plants.

Dr. Grotewold is an Associate Professor of Plant Biology at The Ohio State University. He recieved his Ph.D from the Instituto de Ingenieria Genetics y Bologia Molecular, University of Buenos Aires, Argentina in 1988. His research focuses on Regulation of Gene Expression; Function and Evolution of Myb Proteins; Plant Metabolic Engineering. Combining molecular, cellular, biochemical and genetic approaches, his laboratory investigates fundamental questions regarding the mechanisms by which plants control gene expression, and how transcription factors have impacted plant evolution and speciation. He has started to develop regulatory networks in Arabidopsis that should help explain how the 28,000+ Arabidopsis genes are expressed. In addition, he is utilizing his extensive knowledge on transcription factors for plant metabolic engineering.

"...provides in a single volume most of the commonly used approaches in plant genomics...worth reading and should interest a large public." - Proteomics

"This well written and illustrated guide reads easily and provides detailed protocols and an up-to-date bibliography for all reviewed strategies, making it the book that everybody in this field would need." - Cell Biochem Funct