Plant Innate Immunity

Preface...Table of Contents...Contributing Authors... 1 Using Bioinformatics and Molecular Biology to Streamline Construction of Effector Libraries for Phytopathogenic Pseudomonas syringae Strains Jay Jayaraman, Morgan K. Halane, Sera Choi, Honour C. McCann, and Kee Hoon Sohn 2 Immunoprecipitation Under Non-Denaturing or Denaturing Conditions of Lysine- Acetylated Proteins Expressed in planta Maxime Escouboué and Laurent Deslandes 3 Protein Acetylation in Pathogen Virulence and Host Defense: In Vitro Detection of Protein Acetylation by Radiolabeled Acetyl CoenzymeA Karl J. Schreiber and Jennifer D. Lewis 4 A Novel Assay Based on Confocal Microscopy to Test for Pathogen Silencing Suppressor Functions Mustafa Adhab and James E. Schoelz 5 Quantification of Extracellular ATP in Plant Suspension Cell Cultures Sowmya R. Ramachandran, Sonika Kumar, and Kiwamu Tanaka 6 Measuring Pectin Properties to Track Cell Wall Alterations during Plant-Pathogen Interactions Gerit Bethke and Jane Glazebrook 7 Method to Study Dynamics of Membrane-Bound Plant Transcription Factors during Biotic Interactions in Tomato Supriyo Chowdhury, Payel Bhattacharjee, Shrabani Basak, Shreya Chowdhury, and Pallob Kundu 8 Preparation of Plant Material for Analysis of Protein-Nucleic Acid Interactions by FRET- FLIM Maxime Escouboué, Laurent Camborde, Alain Jauneau, Elodie Gaulin, and Laurent Deslandes 9 Analysis of DNA Methylation Profile in Plants by Chop-PCRPratiti Dasgupta and Shubho Chaudhuri 10 Change in Nucleosome Dynamics during Stress Responses in Plants Amit Paul and Shubho Chaudhuri 11 Measuring Cell Ploidy Level in Arabidopsis thaliana by Flow Cytometry Leiyun Yang, Zhixue Wang, and Jian Hua 12 A Method for Investigating the Pseudomonas syringae-Arabidopsis thaliana Pathosystem under Various Light Environments Daniel L. Leuchtman, Anthony D. Shumate, Walter Gassmann, and Emmanuel Liscum 13 Isolation and Characterization of Plant Metabolite Signals that Induce Type III Secretion by the Plant