Plant MAP Kinases

Transient Expression in Arabidopsis Leaf Mesophyll Protoplast System for Cell-based Functional Analysis of MAPK Cascades Signaling.- Quantification of Stress Induced Mitogen-activated Protein Kinase Expressional Dynamic using Quantitative Real-time PCR.- Analysis of MAPK Activities using MAPK-specific Antibodies.- Detection of Protein Phosphorylation and Charge Isoforms using Vertical one-Dimensional Isoelectric Focusing Gels.- Affinity-based SDS PAGE Identification of Phosphorylated Arabidopsis MAPKs and Substrates by Acrylamide Pendant Phos-Tag(TM).- Identification of Constitutively Active AtMPK6 Mutants using a Functional Screen in Saccharomyces Cerevisiae.- Virus-induced Gene Silencing in Plant MAPK Research.- RNA Interference of Plant MAPK Cascades for Functional Studies.- Immunofluorescent Localization of MAPKs and Colocalization with Microtubules in Arabidopsis Seedling Whole Mount Probes.- Immunofluorescent Localization of MAPKs in Steedman's Wax Sections.- Fluorescent Protein Tagging of Arabidopsis MAPKs for In Vivo Localization Studies.- Bimolecular Fluorescent Complementation (BiFC) by MAP Kinases and MAPK Phosphatases.- Determination of Phosphorylation Sites in Microtubule Associated Protein MAP65-1.- In vivo Phosphorylation of WRKY Transcription Factor by MAPK.- Rapid Mutagenesis-based Analysis of Phosphorylation Sites in Mitogen-activated Protein Kinase Substrates.- Yeast Two-hybrid System for Dissecting the Rice MAPK Interactome.- Experimental and Analytical Approaches to Characterize Plant Kinases using Protein Microarrays.