Practical Handbook on Soil Protists

Soil sampling, processing and storage.- Initial observation of protist from soil.- Abundance calculation of naked and testate amoebae from soil.- Abundance calculation of mycophagous amoebae.- Abundance calculation of ciliates from soilAbundance calculation of flagellates from soil.- Methodology to study species diversity of naked amoeba and testate amoeba.- Non-flooded Petri plate method to study the species diversity of flagellates.- Enumeration of testate amoeba through direct count from soil.- Most Probable Number (MPN) method for enumeration of soil protists.- Standard solutions and media used for isolation of soil protists.- Isolation techniques for amoeba.- Isolation of ciliates and flagellates from soil.- Isolation and Enumeration of Mycophagous Protist.- Media used for enrichment of soil protists.- Enrichment of naked amoebae species.- Enrichment of ciliates and flagellates.- Enrichment of mycophagous protists.- Mono-axenic cultivation of protists.- Axenic cultivation of soil protists.- Classical wet mount method for observing live protists.- SEM: Sample Preparation, Fixation and Staining of Protists.- TEM: Sample Preparation, Fixation and Staining of Protists.- Protargol staining technique for identification of soil protists.-FLUTAX Staining of cilliates.- DNA extraction from trophozoites and cysts using manual protocol.- DNA isolation from single ciliate isolate.- Species identification through sequencing.- DNA Barcoding techniques for Protist.- Unequivocal identification through fluorescence in situ hybridization (FISH) technique.- Isolation, DNA extraction, amplification, and gel electrophoresis of single-celled non-marine foraminifera (Rhizaria).- Isolation and cultivation and staining of Paramecium.- Isolation and cultivation of Euglena.