Retinal Degeneration

Identification and Analysis of Inherited Retinal Disease Genes.-Mouse Models for Studies of Retinal Degeneration and Diseases.-Retinal Fundus Imaging in Mouse Models of Retinal Diseases.-Functional Phenotyping of Mouse Models with ERG.-Phenotyping of Mouse Models with OCT.-Light Damage as a Model of Retinal Degeneration.-N-Methyl-D-Aspartate (NMDA)-mediated Excitotoxic Damage - A Mouse Model of Acute Retinal Ganglion Cell Damage.-Generation of Transgenic X. laevis Models of Retinal Degeneration.-Analysis of Photoreceptor Degeneration in the Zebrafish Danio rerio.-Analysis of Optokinetic Response in Zebrafish by Computer Based Eye Tracking.-Analysis of the Drosophila Compound Eye with Light and Electron Microscopy.-Cell Specific Markers for the Identification of Retinal Cells by Immunofluorescence Microscopy.-A Method of Horizontally-Sliced Preparation of the Retina.-Detection of DNA Fragmentation in Retinal Apoptosis by TUNEL.-High-throughput RNA in situ Hybridization in Mouse Retina.- Assessment of Mitochondrial Damage in Retinal Cells and Tissues using Quantitative Polymerase Chain Reaction for Mitochondrial DNA Damage and Extracellular Flux Assay for Mitochondrial Respiration Activity.-Analysis of Photoreceptor Rod Outer Segment Phagocytosis by RPE Cells in situ.-Ca2+ Microfluorimetry in Retinal Müller Glial Cells.-Functional Analysis of Retinal Microglia and their Effects on Progenitors.-Analysis of Photoreceptor Outer Segment Phagocytosis by RPE Cells in Culture.-Ca2+ Imaging Techniques to Analyze Ca2+ Signaling in Cells and to Monitor Neuronal Activity in the Retina.-Double Chromatin Immunoprecipitation:Analysis of Target Co-Occupancy of Retinal Transcription Factors.-Quantifying the Activity of cis-regulatory Elements in the Mouse Retina by Explant Electroporation.-Optimized Technique for Subretinal Injections in Mice.-Adeno Associated Viral Vectors for Gene Therapy of Inherited Retinal Degenerations.-Barrier Modulation in Drug Delivery to the Retina.

From the book reviews:

"This step-by-step guide to studying retinal cell biology in health and disease covers a large spectrum of techniques and animal model species. ... Certainly, this book is for basic scientists. It is a great tool for those based in laboratories, and researchers at all levels can benefit from the basics to key tips on troubleshooting cutting-edge techniques and avoiding pitfalls. ... This book is great in simplifying new cutting-edge techniques in studying retinal degeneration." (Ayham Skaf, Doody's Book Reviews, May, 2013)

From the book reviews:"This step-by-step guide to studying retinal cell biology in health and disease covers a large spectrum of techniques and animal model species. ... Certainly, this book is for basic scientists. It is a great tool for those based in laboratories, and researchers at all levels can benefit from the basics to key tips on troubleshooting cutting-edge techniques and avoiding pitfalls. ... This book is great in simplifying new cutting-edge techniques in studying retinal degeneration." (Ayham Skaf, Doody's Book Reviews, May, 2013)