SNAREs

Molecular Dynamics Simulations of the SNARE Complex.- Mesoscale Computational Modelling of Protein-membrane Interactions based on Continuum Mean-field Theory.- EPR Lineshape Analysis to IOnvestigate the SNARE Folding Intermediates.- Dynamic Light Scattering Analysis to Dissect Intermediates of SNARE-Mediated Membrane Fusion.- Kinetic and Thermodynamic Quantification of Protein Complex Assembly: The Example of SNAREpins.- Single-molecule Optical Tweezers Study of Regulated SNARE Assembly.- Studying Munc18:Syntaxin Interactions using Small-angle Scattering.- Using Force Spectroscopy to Probe Coiled-Coil Assembly and Membrane Fusion.- SNAP25 S-Guanylation and SNARE Complex Formation.- Analysis of the Role of Sec3 in SNARE Assembly and Membrane Fusion.- Use of Microscale Thermophoresis (MST) to Measure Binding Affinities of Components of the Fusion Machinery.- Use of Surface Plasmon Resonance (SPR) to Determine Binding Affinities and Kinetic Parameters Between Components Important in Fusion Machinery.- Determination of Sec18-Lipid Interactions by Liposome Binding Assay.- Using Nanodiscs to Probe Ca2+-dependent Membrane Interaction of Synaptotagmin-1.- Functional reconstitution of Intracellular Vesicle Fusion using Purified SNAREs and Sec1/Munc18 (SM) Proteins.- Assay of Lipid Mixing and Fusion Pore Formation in the Fusion of Yeast Vacuoles.- A nanodisc-cell Fusion Assay with Single Pore Sensitivity and Sub-millisecond Time Resolution.- An In Vitro Assay of trans-SNARE Complex Formation during Yeast Vacuole Fusion using Epitope Tag-free SNAREs.- A Cell-Free Content Mixing Assay for SNARE-Mediated Multivesicular Body-Vacuole Membrane Fusion.- Reconstituted Proteoliposome Fusion Mediated by yeast SNARE-family Proteins.- Real-Time Fluorescence Detection of Calcium Efflux during Vacuolar Membrane Fusion.- Single Molecule Fluorescence Measurement of SNARE-mediated Vesicle Fusion.- Quantifying Intramolecular Protein Conformational Dynamics under Lipid Interaction using smFRET