The Golgi Complex

4D Confocal Imaging of Yeast Organelles.- Imaging the Polarized Sorting of Proteins from the Golgi Complex in Live Neurons.- Imaging Golgi Outposts in Fixed and Living Neurons.- Analysis of Arf1 GTPase-Dependent Membrane Binding and Remodeling Using the Exomer Secretory Vesicle Cargo Adaptor.- STEM Tomography Imaging of Hypertrophied Golgi Stacks in Mucilage-Secreting Cells.- Reconstitution of COPI Vesicle and Tubule Formation.- Reconstitution of Phospholipase A2-Dependent Golgi Membrane Tubules.- Proteomic Characterization of Golgi Membranes Enriched from Arabidopsis Suspension Cell Cultures.- High Content Analysis of the Golgi Complex by Correlative Screening Microscopy.- Activity Detection of GalNAc Transferases by Protein-Based Fluorescence Sensors In Vivo.- In Situ Proximity Ligation Assay (PLA) Analysis of Protein Complexes Formed Between Golgi-Resident, Glycosylation-Related Transporters and Transferases in AdherentMammalian Cell Cultures.- Creating Knock-Outs of Conserved Oligomeric Golgi Complex Subunits Using CRISPR-Mediated Gene Editing Paired with a Selection Strategy Based on Glycosylation Defects Associated with Impaired COG Complex Function.- Reversible Controlled Aggregation of Golgi Resident Enzymes to Assess Their Transport/Dynamics Along the Secretory Pathway.- Assays to Study the Fragmentation of the Golgi Complex During the G2/M Transition of the Cell Cycle.- The Role of Lysophospholipid Acyltransferases in the Golgi Complex.- Methods to Purify and Assay Secretory Pathway Kinases.

WILLIAM J. BROWN works for Saga Software Inc. in Reston, Virginia, as Director of Research andDevelopment for the Sagavista enterprise integration broker.