Tissue Proteomics
Methods and Protocols
(Sprache: Englisch)
This book presents a compilation of methods that detail improved protein and peptide sample preparation and identification. Chapters guide readers through methods for depletion of myofibril-associated proteins, peptide sample preparation in urinary...
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Klappentext zu „Tissue Proteomics “
This book presents a compilation of methods that detail improved protein and peptide sample preparation and identification. Chapters guide readers through methods for depletion of myofibril-associated proteins, peptide sample preparation in urinary proteomics, purification of targeted proteins from native tissues, fractionation strategies for protein analysis, and GeLC-MS as a sample preparation method for sample preparation for proteomics using minimal amount of tissue. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Tissue Proteomics: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
Inhaltsverzeichnis zu „Tissue Proteomics “
1. Depletion of Myofibril-Associated Proteins Using Selective Protein Extraction as a Tool in Cardiac ProteomicsAchim Treumann, Pawel Palmowski, Wing Chiu Tong, Julie Taggart, Nick Morrice, G. Nicholas Europe-Finner, and Michael J. Taggart 2. Discovery of Immune Reactive Human Proteins by High Density Protein Arrays and Customized Validation of Potential Biomarkers by ELISA
Tara K. Sigdel and Minnie. M. Sarwal
3. HLA Class I and Class II-Induced Intracellular Signaling and Molecular Associations in Primary Human Endothelial Cells
Nicole Valenzuela, Nwe Nwe Soe, Fang Li, Xiaohai Zhang, Yiping Jin, and Elaine F Reed
4. Characterization of Protein Complexes Using Chemical Cross-linking Coupled Electrospray Mass Spectrometry Timothy D. Cummins and Gopal P. Sapkota
5. Optimization for Peptide Sample Preparation for Urine Peptidomics
Tara K Sigdel, Carrie D Nicora, Wei-Jun Qian, and Minnie M Sarwal
6. Purification of Target Proteins from Native Tissues: CCT Complex from Bovine Testes and PP2Ac from Porcine Brains
Feng Guo, Nathan Wlodarchak, Patrick Menden, and Yongna Xing 7. Integrative Analysis of Proteomics Data to Obtain Clinically Relevant Markers
Nathan Salomonis
8. Untargeted Screening of Urinary Peptides Using Off-line Nano-liquid Chromatography - MALDI-TOF/TOF Mass Spectrometry
François-Ludovic Sauvage, Sébastien Passeron, and Pierre Marquet
9. Identifying Clinically Relevant Proteins for Targeted Analysis in the Development of a Multiplexed Proteomic Biomarker Assay
Emily M. Mackay and Oliver F. Bathe
10. In Situ Hybridization and Double Immunohistochemistry for the Detection of VEGF-A mRNA and CD34/Collagen IV Proteins in Renal Transplant Biopsies
Dejan Dobi and Zoltan G. Laszik
11. LC-SRM-based Targeted Quantification of Urinary Protein Biomarkers
Yuqian Gao, Hui Wang, Carrie D. Nicora, Tujin Shi,
... mehr
Richard D. Smith, Tara Sigdel, Minnie Sarwal, David G. Camp II, and Wei-Jun Qian
12. Fractionation of Soluble Proteins Using DEAE-, SP-, and Phenyl Sepharose Chromatographies for Proteomics
Parhom Towfighi, Jacob Shaw, and Tara Sigdel
13. GeLC-MS: A Sample Preparation Method for Proteomics Analysis of Minimal Amount of Tissue Manousos Makridakis and Antonia Vlahou
14. Targeted Proteomics Verification of Breast Cancer Biomarker Candidates
Iva Procházková, Juraj Lenco, and Pavel Bouchal
15. Combination Strategy of Quantitative Proteomics Uncovers the Related-Proteins of Colorectal Cancer in the Interstitial Fluid of Colonic Tissue from the AOM-DSS Mouse Model
Guixue Hou, Yang Wang, Xiaomin Lou, and Siqi Liu
16. Multiple Reaction Monitoring using Double Isotopologue Peptide Standards for Protein Quantification
Azad Eshghi and Christoph H. Borchers
17. Isobaric Labeling Based LC-MS/MS Strategy for Comprehensive Profiling of Human Pancreatic Tissue Proteome
Chih-Wei Liu and Qibin Zhang
18. MALDI Imaging Mass Spectrometry of N-glycans and Tryptic Peptides from the same Formalin-Fixed, Paraffin-embedded Tissue Section Peggi M. Angel, Anand Mehta, Kim Norris-Caneda, and Richard R. Drake
19. Differential Adipose Tissue Proteomics
Kelly J Shields
20. Quantification of Breast Cancer Protein Biomarkers at Different Expression Levels in Human Tumors
Yi Chen, David Britton,Elizabeth R. Wood,Stephen Brantley, Michelle Fournier, Marek Wloch, Vonetta Williams, Joseph Johnson, Anthony Magliocco, Ian Pike, and John M. Koomen
21. Quantitative Proteomic Analysis of Mass Limited Tissue Samples for Spatially-resolved Tissue Profiling
Paul D. Piehowski, Rui Zhao, Ronald J. Moore, Geremy C. Clair, and Charles Ansong
22. High-throughput Proteomics Analysis of Fresh-frozen Biopsy Tissue Samples using Pressure Cycling Technology Coupled with SWATH Mass Spectrometry
Yi Zhu and Tiannan Guo
23. Straightforward Protocol for Gel-free Proteomic Analysis of Adipose Tissue Yvonne Pasing, Armin Schniers, and Terkel Hansen
24. MALDI Imaging Combined to Laser Microdissection-based Microproteomics for Protein Identification. Application to Intratumor Heterogeneity Studies
Rémi Longuespée, Deborah Alberts, Dominique Baiwir, Gabriel Mazzucchelli, Nicolas Smargiasso, and Edwin De Pauw
12. Fractionation of Soluble Proteins Using DEAE-, SP-, and Phenyl Sepharose Chromatographies for Proteomics
Parhom Towfighi, Jacob Shaw, and Tara Sigdel
13. GeLC-MS: A Sample Preparation Method for Proteomics Analysis of Minimal Amount of Tissue Manousos Makridakis and Antonia Vlahou
14. Targeted Proteomics Verification of Breast Cancer Biomarker Candidates
Iva Procházková, Juraj Lenco, and Pavel Bouchal
15. Combination Strategy of Quantitative Proteomics Uncovers the Related-Proteins of Colorectal Cancer in the Interstitial Fluid of Colonic Tissue from the AOM-DSS Mouse Model
Guixue Hou, Yang Wang, Xiaomin Lou, and Siqi Liu
16. Multiple Reaction Monitoring using Double Isotopologue Peptide Standards for Protein Quantification
Azad Eshghi and Christoph H. Borchers
17. Isobaric Labeling Based LC-MS/MS Strategy for Comprehensive Profiling of Human Pancreatic Tissue Proteome
Chih-Wei Liu and Qibin Zhang
18. MALDI Imaging Mass Spectrometry of N-glycans and Tryptic Peptides from the same Formalin-Fixed, Paraffin-embedded Tissue Section Peggi M. Angel, Anand Mehta, Kim Norris-Caneda, and Richard R. Drake
19. Differential Adipose Tissue Proteomics
Kelly J Shields
20. Quantification of Breast Cancer Protein Biomarkers at Different Expression Levels in Human Tumors
Yi Chen, David Britton,Elizabeth R. Wood,Stephen Brantley, Michelle Fournier, Marek Wloch, Vonetta Williams, Joseph Johnson, Anthony Magliocco, Ian Pike, and John M. Koomen
21. Quantitative Proteomic Analysis of Mass Limited Tissue Samples for Spatially-resolved Tissue Profiling
Paul D. Piehowski, Rui Zhao, Ronald J. Moore, Geremy C. Clair, and Charles Ansong
22. High-throughput Proteomics Analysis of Fresh-frozen Biopsy Tissue Samples using Pressure Cycling Technology Coupled with SWATH Mass Spectrometry
Yi Zhu and Tiannan Guo
23. Straightforward Protocol for Gel-free Proteomic Analysis of Adipose Tissue Yvonne Pasing, Armin Schniers, and Terkel Hansen
24. MALDI Imaging Combined to Laser Microdissection-based Microproteomics for Protein Identification. Application to Intratumor Heterogeneity Studies
Rémi Longuespée, Deborah Alberts, Dominique Baiwir, Gabriel Mazzucchelli, Nicolas Smargiasso, and Edwin De Pauw
... weniger
Bibliographische Angaben
- 2018, 2. Aufl., XII, 320 Seiten, 44 farbige Abbildungen, Maße: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Minnie M. Sarwal, Tara K. Sigdel
- Verlag: Springer, Berlin
- ISBN-10: 1493978527
- ISBN-13: 9781493978526
- Erscheinungsdatum: 18.08.2018
Sprache:
Englisch
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